EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of translational regulation by 2 dna binding proteins






Journal of Molecular Biology 173(2): 211-226

Specificity of translational regulation by 2 dna binding proteins

The gene V protein of the filamentous phages fl, fd and M13, and the gene 32 protein of phage T4 share the property of binding strongly and co-operatively to single-stranded nucleic acids, especially DNA. Both are capable of repressing the translation of specific mRNA (gene 32 protein its own and gene V protein that of the filamentous phage gene II), in vivo and in vitro. If the mechanism of repression by either of these proteins were based solely on its ability to bind single strands co-operatively, then the other would be expected to mimic or interfere with its effect in vitro. No such mimicry or interference was found, even at protein concentrations high enough to have substantial non-specific effect on translation. This suggests that the sites of repression on the mRNA must offer something other than simple unstructuredness for binding and repression to occur.


Accession: 006459163



Related references

Fulford, W.; Model, P., 1984: Specificity of translational regulation by two DNA-binding proteins. The gene V protein of the filamentous bacteriophages fl, fd and M13, and the gene 32 protein of bacteriophage T4 share the property of binding strongly and co-operatively to single-stranded nucleic acids, especially DNA. Moreover, both are capable...

Jozwik, C.E.; Miller, E.S., 1992: Regions of bacteriophage T4 and RB69 RegA translational repressor proteins that determine RNA-binding specificity. RegA protein of T4 and related bacteriophages is a highly conserved RNA-binding protein that represses the translation of many phage mRNAs that encode enzymes involved in DNA metabolism. RB69, a T4-related bacteriophage, has a unique regA gene, wh...

Hörnberg, H.; Holt, C., 2013: RNA-binding proteins and translational regulation in axons and growth cones. RNA localization and regulation play an important role in the developing and adult nervous system. In navigating axons, extrinsic cues can elicit rapid local protein synthesis that mediates directional or morphological responses. The mRNA repertoi...

Clemens, M.J., 2001: Translational regulation in cell stress and apoptosis. Roles of the eIF4E binding proteins. Several mechanisms have been identified by which protein synthesis may be regulated during the response of mammalian cells to physiological stresses and conditions that induce apoptotic cell death (reviewed in Clemens et el., Cell Death and Differ...

Clemens, M.J., 2002: Translational regulation in cell stress and apoptosis. Roles of the eIF4E binding proteins. Several mechanisms have been identified by which protein synthesis may be regulated during the response of mammalian cells to physiological stresses and conditions that induce apoptotic cell death (reviewed in Clemens et al., Cell Death and Differ...

Aoyama, T.; Fukao, A.; Fujiwara, T., 2016: Translational regulation is mediated by the cross-talk between the miRNA pathway and RNA binding proteins. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica 147(6): 346-350

Williams R.S., 1986: Coordinate regulation of nuclear genes encoding mitochondrial proteins during mitochondrial proliferation in skeletal muscle evidence that both pre translational and translational or post translational mechanisms are involved. Clinical Research: 633a

Tanzi R.E.; Hyman B.T., 1991: Translational regulation of the amyloid beta protein precursor app via rna binding proteins effects of an alzheimers mutation. Society For Neuroscience Abstracts. 17(1-2): 911

Sanchez, R.; Ingledue, T.; Dominski, Z.; Marzluff, W., 1999: Translational regulation of histone mRNA during early Xenopus development is mediated by two stem loop binding proteins. FASEB Journal 13(7): A1461, April 23

Adeli, K., 2012: Translational control mechanisms in metabolic regulation: critical role of RNA binding proteins, microRNAs, and cytoplasmic RNA granules. Regulated cell metabolism involves acute and chronic regulation of gene expression by various nutritional and endocrine stimuli. To respond effectively to endogenous and exogenous signals, cells require rapid response mechanisms to modulate transc...