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Specificity of trypsin. Cleavage of aspartic acid 101 derivatives of lysozyme by trypsin

, : Specificity of trypsin. Cleavage of aspartic acid 101 derivatives of lysozyme by trypsin. Journal of Biological Chemistry 257(21): 12559-12562

The .beta.-carboxyl group of Asp 101 of hen egg white lysozyme was modified with several amine nucleophiles by the amine-carbodiimide procedure, and the trypsin susceptibilities of the derivatives were analyzed by peptide mapping. The (aminoethyl)asparaginyl peptide bond was completely cleaved by trypsin but the (3-aminopropyl)asparaginyl bond was not. When the positive charge was in the proper position, large residues such as {2-[imidazol-4(5)-ylmethylamino]ethyl} asparagine or (11-amino-3,6,9-triazaundecyl)asparagine were susceptible to trypsin.

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