+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of tyrosine protein kinases of the structurally related receptors for insulin and insulin like growth factor i tyrosine containing synthetic polymers as specific inhibitors or substrates

, : Specificity of tyrosine protein kinases of the structurally related receptors for insulin and insulin like growth factor i tyrosine containing synthetic polymers as specific inhibitors or substrates. Archives of Biochemistry & Biophysics 260(1): 416-426

The receptors for insulin and insulin-like growth factor (IGF) I are structurally similar transmembrane proteins. Ligand binding to the extracellular domain of the receptor stimulates its cytoplasmic tyrosine protein kinase which phosphorylates its own .beta. subunit as well as exogenous substrates. It is believed, from several lines of evidence, that tyrosine-specific protein kinases are mediating some or all of the actions of insulin (or IGF-I). In order to gain insights into the substrate specificity of the structurally related insulin and IGF-I receptor kinases, we have studied the action of highly purified receptors isolated from human placental membranes. Present studies using selected tyrosine-containing polymers have revealed: (i) Polymers such as (Y,A,E)n and (Y-A-E)n inhibit .beta. subunit autophosphorylation and exogenous substrate phosphorylation by autophosphorylated receptors. (ii) Insulin receptor kinase is at least 10 times more sensitive to these inhibitors than IGF-I receptor kinase. (iii) (Y-A-E)n is .apprx. 8 times more potent an inhibitor than (Y,A,E)n toward both receptors. (iv) While (E4, Y1)n and (E6,A3,Y1)n are good substrates for both receptor kinases, the ratio of phosphate incorporation into the former to the latter is characteristically high (.apprx. 4) for the IGF-I receptor and low (.apprx. 1) for the insulin receptor. These results imply that the substrate specificity and enzymatic action of these two receptor kinases are distinct.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006459175

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Jinbo, T.; Shinmura, R.; Shida, T.; Shimizu, M.; Honda, M.; Hayashi, S.; Iguchu, K.; Yamamoto, S., 1997: Experimental detection of canine haemoglobin (occult blood) in canine faeces by reversed passive latex agglutination. A reversed passive latex agglutination test (RPLA) using anti-canine haemoglobin (Hb) antibody was developed for detecting bleeding in the lower digestive organs in dogs, and its applicability as a simple test for faecal occult blood was assessed....

Selim A.K.; Serry M.S.; Satour M.M.; Al Ahmar B.A., 1976: Breeding for disease resistance in sesame sesamum indicum part 3 inheritance of resistance to fusarium oxysporum. Egyptian Journal of Phytopathology 8: 19-24

Hodell, David, A.; Vayavananda, Anon, 1993: Middle Miocene paleooceanography of the western equatorial Pacific (DSDP site 289) and the evolution of Globorotalia (Fohsella). Evolution of the planktic foraminiferal lineage Globorotalia (Fohsella) occurred during the Miocene between 23.7 and 11.8 Ma and forms the basis for stratigraphic subdivision of the early middle Miocene (Zones N10 through N12). Important morpholog...

Ringstrom S.; Schwartz N.B., 1983: Differential control of luteinizing hormone and fsh secretion in male rats. Negro-Vilar, A (Ed ) Male Reproduction And Fertility; International Meeting, Mexico City Xvi+390p Raven Press: New York, N Y , Usa Illus P171-182

Verbeek, A. G.; Mackay, W. C.; Birkholz, D. A., 1996: Ethoxyresorufin-O-deethylase induction in rainbow trout exposed to diluted oil sand wastewater. Canadian Technical Report of Fisheries & Aquatic Sciences 0(2093): 85

Anonymous, 2008: Melamine contamination, China. Weekly Epidemiological Record: 40, 358

Anonymous, 2012: Effects of School-Wide Positive Behavioral Interventions and Supports on Child Behavior Problems. School-Wide Positive Behavioral Interventions and Supports (SWPBIS) is a widely used universal prevention strategy currently implemented in >16 000 schools across the United States. Previous research has shown positive effects on school climate...

Grimaud, C.; Nicoli, N.; Orehek, J.; Guillot, C., 1977: Effect of beta adrenergic stimulation on the closing volume in normal subjects. In 12 normal subjects, the effects were studied of .beta.-adrenergic stimulation (salbutamol, 500 .mu.g inhaled) on the closing volume (CV) and the closing capacity and on the ratios of these indexes to the vital capacity (VC) and the total lung c...

Makus, D. J., 1997: Initial observations on cassava establishment and adaptability in the Rio Grand Valley. Hortscience 32(4): 605

Anonymous, 2012: NASAs Fermi detects the highest-energy light from a solar flare. During a powerful solar blast on March 7, NASA's Fermi Gamma-ray Space Telescope detected the highest-energy light ever associated with an eruption on the sun. The discovery heralds Fermi's new role as a solar observatory, a powerful new...