+ Translate

Specificity of uracil uptake in Neurospora crassa

, : Specificity of uracil uptake in Neurospora crassa. Journal of Bacteriology 139(1): 212-219

The specificity of uracil uptake was investigated in germinating wild-type conidia of Neurospora crassa. From comparative inhibition studies, several generalizations concerning the specificity of uracil uptake can be made. (i) The tautomeric forms of uracil analogs is an important determinant of recognition by the uptake system. (ii) Substituents at the 5 position of the pyrimidine ring may impose steric constraints on binding. (iii) The presence of a negative charge results in the loss of recognition. (iv) The double bond between the 5 and 6 carbons appears to be important for recognition. (v) Purine bases do not inhibit uracil uptake. Crude extracts of the transport-deficient mutant strain uc-5 pyr-1 were shown to have uridine 5'-monophosphate pyrophosphorylase activity comparable to that of the wild-type strain, suggesting that uracil uptake in Neurospora does not occur by a group translocation mechanism involving phosphoribosylation. Specificity studies of uridine 5'-monophosphate pyrophosphorylase indicated that phosphoribosylation was not an important determinant of the specificity of uracil uptake.

(PDF 0-2 workdays service)

Accession: 006459178

PMID: 156718

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Magill, J.M.; Edwards, E.S.; Sabina, R.L.; Magill, C.W., 1976: Depression of uracil uptake by ammonium in Neurospora crassa. The mechanism of uracil uptake and one aspect of its regulation were studied in germinated conidia of Neurospora crassa. Uracil was found to be taken up by a transport mechanism that did not exhibit Michaelis-Menten kinetics. Rather, the kinetic p...

Winkelmann, G.Z.hner, H., 1973: Metabolic products of microorganisms. 115. uptake of iron by Neurospora crassa. 1. to the specificity of iron transport. Archiv fur Mikrobiologie: 8 (1) 49-60

Winkelmann, G.; Zähner, H., 1973: Metabolic products of microorganisms. 115. Uptake of iron by Neurospora crassa. I. To the specificity of iron transport. Archiv für Mikrobiologie 88(1): 49-60

Woodward, V.W.; Munkres, K.D.; Suyama, Y., 1957: Uracil metabolism in Neurospora crassa. The pyrimidine re-quirement of a N. crassa mutant, Pyr-1 (263) can be met partly by all the intermediates produced by reductive degradation of uracil, with the exception of [beta]-alanine. Wild-type Neurospora stores [beta]-ureido-propionic acid a...

Chakraborty, K.P.; Loring, H.S., 1960: Incorporation of uracil-2-C14 into the nucleic acids of Neurospora crassa. To determine whether or not uracil utilization was the only pathway of pyrimidine biosynthesis in the pyrimidine-deficient Neurospora, 1298, the growth-promoting abilities of deoxyuridine, deosycytidine, and thymidine by themselves and in the pres...

Chakraborty, Kamala Prasad, 1960: Studies on growth and incorporation of uracil-2-C14 in Neurospora crassa. Dissertation Absts 20(8): 3044-3045

Nemerofsky, A., 1977: Effect of pre treatment with 5 bromo uracil in an uv sensitive strain of neurospora crassa. 5-Bromouracil enhanced the mutation frequency in an UV sensitive strain of N. crassa (uvs-2) that was previously irradiated with UV light. The design of the experiment eliminated light or dark repair as possible hypotheses to account for the obser...

Nemerofsky A., 1975: The interactive effect of uv irradiation and 5 bromo uracil at the rib 1 locus in neurospora crassa. Canadian Journal of Genetics & Cytology 17(2): 275-278

Wu, J.; Glass, N.L., 2001: Identification of specificity determinants and generation of alleles with novel specificity at the het-c heterokaryon incompatibility locus of Neurospora crassa. The capacity for nonself recognition is a ubiquitous and essential aspect of biology. In filamentous fungi, nonself recognition during vegetative growth is believed to be mediated by genetic differences at heterokaryon incompatibility (het) loci....

Hsu, C.A.; Saewert, M.D.; Polsinelli, L.F.; Abbott, M.T., 1981: Uracil's uncoupling of the decarboxylation of alpha-ketoglutarate in the thymine 7-hydroxylase reaction of Neurospora crassa. Highly purified preparations of thymine 7-hydroxylase from Neurospora crassa catalyzed the decarboxylation of alpha-ketoglutarate but yielded no hydroxylated product when uracil was substituted for thymine in the standard incubation mixture. Altho...