+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of urinary kallikrein activity determination with an amidolytic assay

, : Specificity of urinary kallikrein activity determination with an amidolytic assay. Revista Espanola de Fisiologia 39(3): 225-230

The specificity of a method for the determination of the urinary kallikrein activity based on the amidolytic hydrolysis of the synthetic substrate H-D-valyl-leucyl-arginine-p-nitroanilide has been studied. Results show a good specificity for kallikrein. The amidolytic activity was totally inhibited by aprotinin. The urinary amidolytic activity showed identical dependence on changes in the ionic strength and pH of the incubation medium as the highly purified kallikrein. The sensitivity of the assay was 0.6 nkat/l. The intra-assay coefficient of variation was 4% and the inter-assay coefficient of variation was 10%. The percentage of recovery was between 100 and 110% indicating that no activators or inhibitors of the kallikrein activity on this substrate were present in the urinary samples. Values obtained in presumably healthy subjects were 0.14 .+-. 0.08 pkat/s in man (range: 0.05-0.27 pkat/s) and 0.49 .+-. 0.11 pkat/s in rat (range: 0.35-0.58 pkat/s).

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006459179

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Belokon, Yuri, N.; Bespalova, Natalia, B.; Churkina, Tatiana, D.; Cisarova, Ivana; Ezernitskaya, Marina, G.; Harutyunyan, Syuzanna, R.; Hrdina, Radim; Kagan, Henri, B.; Kocovsky, Pavel; Kochetkov, Konstantin, A.; Larionov, Oleg, V.; Lyssenko, Konstantin, A.; North, Michael; Polasek, Miroslav; Peregudov, Alexander, S.; Prisyazhnyuk, Vladimir, V.; Vyskocil,, 2003: Synthesis of alpha-amino acids via asymmetric phase transfer-catalyzed alkylation of achiral nickel(II) complexes of glycine-derived Schiff bases. Achiral, diamagnetic Ni(II) complexes 1 and 3 have been synthesized from Ni(II) salts and the Schiff bases, generated from glycine and PBP (7) and PBA (11), respectively, in MeONa/MeOH solutions. The requisite carbonyl-derivatizing agents pyridine...

Schuepbach P.; Gaberthuel T.; Guggenheim B.; Lutz F., 1991: Healing events leading to new connective tissue attachment an ultrastructural study. Journal of Dental Research 70(SPEC ISSUE APRIL): 467

Semus S.F.; Martin B.R., 1990: A computergraphic investigation into the pharmacological role of the thc cannabinoid phenolic moiety. There has been much debate as to the nature of the cannabinoid-receptor interaction, whether by traditional interpretation or by means of membrane pertubation. Whichever hypothesis is correct, the structural requirements for pharmacological action...

Afzal S.M.J.; Kesavan P.C., 1979: Influence of seed moisture content and post irradiation hydration temperature on the kinetics of reactivity towards oxygen or decay of oxygen sensitive sites. The rate of development of post-irradiation O2-dependent damage when O2 is available, and its rate of elimination when seeds are first post-hydrated in O2-free water prior to their transfer to oxygenated water, was studied in barley seeds of .appr...

Vogt, Angela, K.; Wrobel, Guenter; Meyer, Wolfgang; Knoll, Wolfgang; Offenhaeusser, Andreas, 2005: Synaptic plasticity in micropatterned neuronal networks. Synaptic plasticity is thought to be of central importance for information processing by the nervous system. Additionally, specific neuronal connectivity patterns in the brain are implicated to play a role in the perception, processing and storage...

Gogia, J. S., 2000: Information technology and logistics efficiency. The role of information systems in the planning, designing, implementation and control of process was examined by elaborating its main characteristics and flow of information. The information system has been integrated with various available tools...

Geng R S.; Miao S H., 1991: Selection and characterization of maize variants resistant to s 2 aminoethyl l cysteine and 5 methyltryptophan. Using tissue culture selection techniques, variants resistant to S-(2-aminoethyl)-L-cysteine (AEC) and 5-methyltryptophan (5MT) were, respectively, isolated from Opaque-2 maize [Zea mays] inbred line "Zhongxi 037/02" and "Zhongxi 09...

Zhang, Ji; Furukawa, Robert D.; Fremes, Stephen E.; Mickle, Donald A. G.; Weisel, Richard D., 1997: Effects of butanedione monoxime and temperature on prolonged cardiac storage. Background: The optimal temperature for cardiac allograft storage remains controversial. We conjectured that supplementation of the potent cardioprotective agent 2,3-butanedione monoxime with calcium may improve allograft storage and make the prec...

Muchmore J.S.; Cooper D.K.C.; Ye Y.; Schlegel V.; Zuhdi N., 1991: Prevention of loss of vertebral bone density in heart transplantation patients. Journal of Heart & Lung Transplantation 10(1 PART 2): 178

Nakada M.; Ida Y., 1983: Theoretical world-wide sea level change associated with non-Newtonian flow in the upper mantle. Pages 99-102: