EurekaMag.com logo
+ Translate

Specificity repertoire of lymphocytes from multiple myeloma patients 1. high frequency of b cells specific for idiotypic and fab' 2 region determinants on immunoglobulin


, : Specificity repertoire of lymphocytes from multiple myeloma patients 1. high frequency of b cells specific for idiotypic and fab' 2 region determinants on immunoglobulin. Journal of Clinical Immunology 5(4): 275-284

The specificity repertoire of B lymphocytes from 14 multiple myeloma patients was studied using the technique of Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes (PBL) coupled with clonal analysis by limiting dilution. It was found that up to 100% of the B cells from myeloma patients undergoing EBV transformation secrete IgM specific for determinants on the F(ab')2 region of autologous and/or heterologous monoclonal Ig. In normal individuals 0.02-0.73% of the transformed B cells secrete IgM specific for F(ab')2 determinants. Two patients with monoclonal gammopathy of undetermined significance had only a weak reactivity to F(ab')2 fragments. The number of anti-F(ab')2 B cells was up to 145-fold greater in patients than in normal donors. The majority of antibodies from patient clones recognized determinants shared among 3-12 different F(ab')2 fragments, whereas those originating from normal donor B cells saw determinants expressed on only 1 or 2 of the panel of test F(ab')2 fragments. There was a preference for autologous M components and a high proportion of antiidiotypic reactivity in 5 of 8 patients so analyzed. These findings may indicate the existence of an anti-F(ab')2 immunoregulatory network mediating patient immunodeficiency, thereby creating an abnormality that may enable the progression of multiple myeloma.

(PDF 0-2 workdays service)

Accession: 006459198

PMID: 2995434

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Pilarski, L.M.; Piotrowska-Krezolak, M.; Gibney, D.J.; Winger, L.; Winger, C.; Mant, M.J.; Ruether, B.A., 1985: Specificity repertoire of lymphocytes from multiple myeloma patients. I. High frequency of B cells specific for idiotypic and F(ab')2-region determinants on immunoglobulin. The specificity repertoire of B lymphocytes from 14 multiple myeloma patients has been studied using the technique of Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes (PBL) coupled with clonal analysis by limiting dilution....

Binz, H.; Wigzell, H., 1975: Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. II. Determination of frequency and characteristics of idiotypic T and B lymphocytes in normal rats using direct visualization. Anti-idiotypic antibodies made against the antigen-binding receptors of T lymphocytes for a given antigen (Ag-B locus antigens in rats) can be shown to react with IgG antibodies of the same antigen-binding reactivity. Using such anti-idiotypic ant...

Topp, M.S.; Rauser, G.; Kroger, N.; Kuntz, G.; Einsele, H., 2002: MUC-1 Specific T-Cells Are Present in Multiple Myeloma Patients at High Frequency after Allogeneic Transplantation but May Not Mediated the Graft Versus Myeloma Effect. Autologous stem cell transplantation (SCT) followed by allogeneic SCT with reduced conditioning regimens can lead to complete remissions in patients with multiple myeloma. The observed high remission rates are most likely associated with an induct...

Binz, H.; Wigzell, H., 1975: Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. III. Physical fractionation of specific immunocompetent T lymphocytes by affinity chromatography using anti-idiotypic antibodies. Anti-idiotypic antibodies made against antigen-binding receptors on T lymphocytes with specificity for certain Ag-B locus antigens selectively react with T lymphocytes with potential immune reactivity against the very same Ag-B antigens. This was...

Rosenstein, R.W.; Zeldis, J.B.; Konigsberg, W.H.; Richards, F.F., 1979: The location and expression of idiotypic determinants in the immunoglobulin variable region--I. Characterization of antibodies directed against the variable region of mouse myeloma immunoglobulins 315 and 460. Molecular Immunology 16(6): 361-370

Rinaldi, M.; Ria, F.; Parrella, P.; Signori, E.; Serra, A.; Ciafrè, S.A.; Vespignani, I.; Lazzari, M.; Farace, M.G.; Saglio, G.; Fazio, V.M., 2001: Antibodies elicited by naked DNA vaccination against the complementary-determining region 3 hypervariable region of immunoglobulin heavy chain idiotypic determinants of B-lymphoproliferative disorders specifically react with patients' tumor cells. Several reports have suggested that the mechanism of protection induced by antiidiotypic vaccination against low-grade lymphoproliferative disorders is likely to be antibody mediated. Here we test the hypothesis that DNA vaccination with the short...

Abbas, A.K.; Perry, L.L.; Bach, B.A.; Greene, M.I., 1980: Idiotype-specific T cell immunity. I. Generation of effector and suppressor T lymphocytes reactive with myeloma idiotypic determinants. Journal of Immunology 124(3): 1160-1166

Pilarski, L.M.; Ruether, B.A.; Mant, M.J., 1985: Abnormal function of B lymphocytes from peripheral blood of multiple myeloma patients. Lack of correlation between the number of cells potentially able to secrete immunoglobulin M and serum immunoglobulin M levels. Multiple myeloma patients are deficient in normal polyclonal serum immunoglobulins. To determine the reasons for this decrease, we quantitated and compared the number of surface IgM+ B lymphocytes, and the number of B cells susceptible to transfor...

Nguyen-Pham, T-Nhan.; Im, C-Min.; Nguyen, T-Anh.Thi.; Lim, M-Seon.; Hong, C.Yi.; Kim, M-Hyun.; Lee, H.Ju.; Lee, Y-Kyung.; Cho, D.; Ahn, J-Sook.; Yang, D-Hwan.; Kim, Y-Kyeoung.; Chung, I-Joo.; Kim, H-Joon.; Lee, J-Jung., 2011: Induction of myeloma-specific cytotoxic T lymphocytes responses by natural killer cells stimulated-dendritic cells in patients with multiple myeloma. The interaction between dendritic cells (DCs) and natural killer (NK) cells plays a key role in inducing DC maturation for subsequent T-cell priming. We investigated to generate potent DCs by stimulated with NK cells to induce myeloma-specific cyt...

Binz, H.; Wigzell, H., 1975: Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. IV. Isolation of two groups of naturally occurring, idiotypic molecules with specific antigen-binding activity in the serum and urine of normal rats. In the rat the major histocompatibility locus antigens are determined by the Ag-B locus. In the present article evidence is presented that the sera and urine of normal adult rats contain naturally occurring antibody-like molcules with reactivity t...