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Specificity repertoire of splenic lyt 2 positive f23 positive cytotoxic lymphocyte precursors from b6 mice


, : Specificity repertoire of splenic lyt 2 positive f23 positive cytotoxic lymphocyte precursors from b6 mice. Cellular Immunology 106(1): 100-113

As revealed by flow cytometric analysis, about 30% of nylon wool nonadherent Lyt-2+ B6 spleen cells were F23+, i.e., were stained with the monoclonal antibody F23, 1 directed against an allotypic T-cell receptor determinant. The specificity repertoire of splenic Lyt-2+/F23+ cytotoxic lymphocyte precursors (CLP) from B6 mice was investigated in a limiting dilution (LD) system designed to support clonal expansion in vitro of a representative fraction of this T-cell subset: in highly purified Lyt-2+ responder cells cocultured with mitomycin-treated F23 hybridoma cells in the presence of (recombinant) interleukin 2 under LD conditions, one out of three Lyt-2+/ F23+ CLP gave rise to a functional cytotoxic T lymphocyte (CTL) clone. The split-well analysis of individual CTL populations demonstrated a clear-cut segregation of the lytic reactivities toward different allogeneic Con A blast targets. A large fraction of B6-derived CTL clones (3-10%) specifically lysed fully H-2 allogeneic (H-2k, H-2d), or H-2K mutant (bm 1) targets. Self-reactive and allorestricted lytic patterns were not found.

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Related references

Reimann J.; Bellan A.; Kabelitz D., 1986: Specificity repertoire of splenic lyt2 positive f23 positive cytotoxic lymphocyte precursors from b6 mice activated under limiting dilution conditions. Immunobiology 173(2-5): 368

Reimann, J.; Bellan, A.; Kabelitz, D., 1987: Specificity repertoire of splenic Lyt-2+/F23+ cytotoxic lymphocyte precursors from B6 mice. As revealed by flow cytometric analysis, about 30% of nylon wool nonadherent Lyt-2+ B6 spleen cells were F23+, i.e., were stained with the monoclonal antibody F23.1 directed against an allotypic T-cell receptor determinant. The specificity reperto...

Anonymous, 1986: Use of v beta8 genes in splenic lyt 2 positive cytotoxic lymphocyte precursors reactive to bm1 or bm14 alloantigen in individual b6 mice

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Oshimi K.; Oshimi Y.; Yamada O.; Wada M.; Hara T.; Mizoguchi H., 1990: Cytotoxic t lymphocyte triggering via cd16 is regulated by cd3 and cd8 antigens studies with t cell receptor tcr alpha beta positive cd3 positive 16 positive and tcr gamma delta positive cd3 positive 16 plus granular lymphocytes. The role of CD3 and CD8 Ag in CD16-mediated CTL triggering was studied in TCR-.alpha.beta.+ and TCR-.gamma.delta.+ granular lymphocytes (GL). The TCR-.alpha.beta.+/CD3+4-8+16+ GL obtained from patients with GL-proliferative disorders, antibody-dep...

Wagner H.; Hardt C., 1986: Heterogeneity of the signal requirements during the primary activation of resting lyt 2 positive cytotoxic t lymphocyte precursors into clonally developing cytotoxic t lymphocyte. Fleischer, B , J Reimann And H Wagner (Ed ) Current Topics in Microbiology And Immunology, 126 Specificity And Function Of Clonally Developing T Cells; Workshop, Schloss Reisensburg, West Germany, Mar 17-20, 1985 Xv+316p Springer-Verlag: New York, N Y , Usa; Berlin, West Germany Illus 143-154