+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity spectrum and cell surface markers of mono functional and multi functional mixed leukocyte culture derived t cell clones in man

Specificity spectrum and cell surface markers of mono functional and multi functional mixed leukocyte culture derived t cell clones in man

European Journal of Immunology 12(7): 607-615

Alloactivated human lymphocytes were cloned by limiting dilution followed by expansion of clonal progeny using interleukin 2. Their differing functional specificities were analyzed in terms of reactivity in primed lymphocyte typing (PLT), cell-mediated cytotoxicity to normal peripheral blood mononuclear cells or to cell line targets susceptible to lysis by natural killer cells, and suppressive capacity in mixed leukocyte cultures. T lymphocyte clones mediating exclusively PLT, CML [cell-mediated lympholysis] or suppression were isolated. All clones with natural killer-like activity were multifunctional in that they strongly suppressed mixed leukocyte cultures and had variable, although generally weak, activity in PLT and CML. From a total of 148 clones initially screened, about half manifested PLT reactivity. Of these, 60% were restimulated exclusively by determinants associated with the HLA-D type of the original priming cells, whereas the remainder were restimulated by a variety of different HLA-D and HLA non-D products. Similar heterogeneity of responses at the clonal level was observed among the populations active in CML. Using monoclonal antibodies, surface marker phenotyping of functionally distinct clones was undertaken in an attempt to correlate surface marker profiles with particular functions. All clones tested were mature, activated T cells (Lyt-3+, T28+, OKT3+, HLA-DR+, HTA1-, OKT6-). Many of the clones described here, whether functional in PLT CML, having natural killer-like or suppressor cell activity, expressed putative helper/inducer T cell markers defined by OKT4. The putative suppressor/cytotoxic marker OKT8 appeared on some natural killer-functional clones, but not on CML or suppressor cell clones. Clones with natural killer-like function were identifiable by their additional unique expression of the antigen detected by the rat monoclonal antibody YD1/48.HLK; however, the monoclonal antibody M522 reacted selectively with clones having natural killer or CML function, but not with those with PLT or suppressor cell function. Cloned T cells with PLT, CML, natural killer or suppressor activities, derived from MLC [mixed leukocyte culture], can act as valuable tools in the elucidation of T cell function in vitro. The correlation of defined function with analysis of cell surface markers using monoclonal antibodies will be of value in dissecting components of human immune responses and determining the functional relevance, if any, of structures detected by such antibodies.

(PDF emailed within 1 workday: $29.90)

Accession: 006459205

Download citation: RISBibTeXText

Related references

Specificity spectrum and cell surface markers of mono- and multi-functional mixed leukocyte culture-derived T cell clones in man. European Journal of Immunology 12(7): 607-615, 1982

Clones of human cytotoxic T lymphocytes derived from an allosensitized individual: HLA specificity and cell surface markers. Scandinavian Journal of Immunology 14(3): 213-224, 1981

Functional properties and cell surface markers of murine cytolytic thymus derived cell lines. Journal of Supramolecular Structure 8(SUPPL 3): 302, 1979

Murine cytolytic thymus derived cell lines stability of functional phenotype and expression of cell surface markers. Bach, F H Et Al (Ed ) Icn-University Of California, Los Angeles Symposia on Molecular And Cellular Biology, Vol 16 T And B Lymphocytes Recognition And Function; Keystone, Colo , Usa, Mar 25-30, 1979 Xxi+709p Academic Press: New York, N Y , Usa; London, England Illus P679-688, 1979, 1980

Long-term culture, cloning, and surface markers of mixed leukocyte culture-derived human T lymphocytes with natural killer-like cytotoxicity. Journal of Immunology 128(4): 1892-1896, 1982

Mixed lymphocyte culture derived human helper factors that promote bone marrow derived cell differentiation induction functional characterization and role of ia antigens. Journal of Immunology 124(6): 2930-2935, 1980

Noncytotoxic T cell clones obtained from a human mixed leukocyte culture. Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 23(1): 1-11, 1990

Stimulation in the mixed leukocyte culture and generation of effector cells in cell mediated lympholysis by a human thymus derived lympho blast cell line. Cellular Immunology 19(2): 276-281, 1975

Functional activity in vivo of effector t cell populations 1. anti tumor activity exhibited by allogeneic mixed leukocyte culture cells. Journal of Immunology 129(3): 1292-1298, 1982

Functional gamma chain-associated T cell receptors on cerebrospinal fluid-derived natural killer-like T cell clones. Journal of Experimental Medicine 165(5): 1453-1458, 1987

Human Tc1 and Tc2/Tc0 CD8 T-cell clones display distinct cell surface and functional phenotypes. Blood 95(1): 231-240, 1999

Phenotypic and functional properties of murine gamma delta T cell clones derived from malaria immunized, alpha beta T cell-deficient mice. International Immunology 8(3): 359-366, 1996

Mixed leukocyte culture vs cell mediated lympholysis in a case of thymus derived cell immuno deficiency and adenosine deaminase deficiency. Bergsma, Daniel (Ed ) National Foundation-March Of Dimes Birth Defects Original Article Series, Vol 11, No 1 Immunodeficiency in Man And Animals Workshop St Petersburg Beach, Fla , U S A , Feb 4-8, 1973 Xx+599p Illus Sinauer Associates, Inc : Sunderland, Mass , U S A Isbn 0-87893-206-2 486-488, 1975

Surface markers on human bone marrow and thymus derived lymphocytes part 6 cyto toxicity against cell lines as a functional marker for lymphocyte sub populations. International Journal Of Cancer: 596-605, 1975