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Specificity study of purified protein derivative reactive human t cell line and clones

, : Specificity study of purified protein derivative reactive human t cell line and clones. Immunology Letters 9(2-3): 81-86

Peripheral blood mononuclear cells from a PPD [purified protein derivative] sensitive human subject, in vitro stimulated by PPD, could be maintained for several weeks in interleukin 2-containing medium, with regular reactivation with PPD and irradiated autologous mononuclear cells used as antigen-presenting cells. The proliferative response of this T cell PPD-reactive bulk culture could be stimulated to proliferated by PPD itself as well as by BCG and other mycobacteria. This T cell line comprised a majority of T cells expressing the OKT4-, OKT8 phenotype, and a minor proportion of T cells (8%) showing the OKT4. OKT8- phenotype. Two PPD-reactive clones, both OKT4-, were obtained from this line by the limiting dilution technique. Clone C9 was found to be highly specific of PPD and BCG whereas clone A9 could be activated by 4 other species of mycobacteria [mycobacterium kansasii, M. fortuitum, M. triviale and M. smegmatis]. These data demonstrate the existence of species-specific and cross-reactive epitopes within PPD and prompt for the use of PPD-reactive clones as a tool for identification and purification of the molecules bearing these epitopes.

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