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Spectinomycin resistance at site 1192 in 16s ribosomal rna of escherichia coli an analysis of three mutants


, : Spectinomycin resistance at site 1192 in 16s ribosomal rna of escherichia coli an analysis of three mutants. Biochimie (Paris) 69(8): 885-890

Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of Escherichia coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transformed cells but none affected the growth rate in the absence of the antibiotic. The G-1192 mutant conferred remarkable resistance, permitting growth in 40 mg/ml of spectinomycin.

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