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Spectinomycin resistance at site 1192 in 16s ribosomal rna of escherichia coli an analysis of three mutants


, : Spectinomycin resistance at site 1192 in 16s ribosomal rna of escherichia coli an analysis of three mutants. Biochimie (Paris) 69(8): 885-890

Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of Escherichia coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transformed cells but none affected the growth rate in the absence of the antibiotic. The G-1192 mutant conferred remarkable resistance, permitting growth in 40 mg/ml of spectinomycin.

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Makosky, P.C.; Dahlberg, A.E., 1987: Spectinomycin resistance at site 1192 in 16S ribosomal RNA of E. coli: an analysis of three mutants. Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of E. coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transfor...

Dekio, S.; Takata, R.; Osawa, S.; Tanaka, K.; Tamaki, M., 1970: Genetic studies of the ribosomal proteins in Escherichia coli. IV. Pattern of the alteration of ribosomal protein components in mutants resistant to spectinomycin or erythromycin in different strains of Escherichia coli. Molecular & General Genetics 107(1): 39-49

Dekio, S.; Takata, R.; Osawa, S.; Tanaka, K.; Tamaki, M., 1970: Genetic studies of the ribosomal proteins in escherichia coli part 4 pattern of the alteration of ribosomal protein components in mutants resistant to spectinomycin or erythromycin in different strains of escherichia coli. Molecular and General Genetics 107(1): 39-49

Dekio, S.; Takata, R., 1969: Genetic studies of the ribosomal proteins in Escherichia. coli. II. Altered 30s ribosomal protein component specific to spectinomycin resistant mutants. Molecular & General Genetics 105(3): 219-224

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Mark L.G.; Sigmund C.D.; Morgan E.A., 1983: Spectinomycin resistance due to a mutation in an ribosomal rna operon of escherichia coli. A spectinomycin resistance mutation was isolated in an E. coli rRNA operon (rrnH) located on a multicopy plasmid. Cell-free protein-synthesizing extracts made from cells containing the plasmid were partially resistant to spectinomycin. Although sp...

Funatsu, G.; Schiltz, E.; Wittmann, H.G., 1972: Ribosomal proteins part 27 localization of the amino acid exchanges in protein s 5 from 2 escherichia coli mutants resistant to spectinomycin. Molecular and General Genetics 114(2): 106-111

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Dombou, M.; Mizuno, T.; Mizushima, S., 1977: Interaction of the cytoplasmic membrane and ribosomes in Escherichia coli; altered ribosomal proteins in sucrose-dependent spectinomycin-resistant mutants. Alterations in the ribosomes of sucrose-dependent spectinomycin-resistant (Sucd-Spcr) mutants of Escherichia coli were studied. Subunit exchange experiments showed that 30S subunits were responsible for the resistance of ribosomes to spectinomycin...

Andrésson, O.S.; Magnúsdóttir, R.A.; Eggertsson, G., 1976: Deletions of ribosomal protein genes in Escherichia coli merodiploids heterozygous for resistance to streptomycin and spectinomycin. In a merodiploid strain of Escherichia coli heterozygous for the ribosomal protein genes spc and str, deletions were observed preventing the expression of either gene but permitting the expression of the other. This suggests that the spc and str g...