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Spectra membrane binding and potentiometric responses of new charge shift probes


, : Spectra membrane binding and potentiometric responses of new charge shift probes. Biochemistry 24(21): 5749-5755

The properties of a series of new potentiometric membrane probes have been explored. The probes all contain an (aminostyryl)pyridinium chromophore or a more highly conjugated analogue. The spectral properties of the dyes are discussed in terms of the excitation-induced charge shift from the pyridine to the aniline; this charge shift also provides the basis for the voltage dependence of the spectra according to an electrochrome mechanism. The spectral responses to a membrane potential of a hemispherical bilayer have been obtained and, grossly, are quite similar for all probes tested. The more subtle variations from dye to dye can be partially rationalized by consideration of binding parameters, the depth within the membrane, and structural factors. The most potential sensitive dye in this collection has been designated di-4-ANEPPS and a 6-amino-2-naphthyl group in place of the p-anilino on the parent chromophore. Both the relative fluorescence emission and excitation responses have maxima of 8% per 100 mV, and these two spectra display a striking symmetry.

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Accession: 006459340

PMID: 4084490

DOI: 10.1021/bi00342a010

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Other references

Vrbovský, L.; Kubácek, L., 1978: Approximating the critical dose in chronic toxicologic experiment. Bratislavske Lekarske Listy 70(4): 484-493

Ruder, A.M.; Waters, M.A.; Butler, M.Ann.; Carreón, T.; Calvert, G.M.; Davis-King, K.E.; Schulte, P.A.; Sanderson, W.T.; Ward, E.M.; Connally, L.Barbara.; Heineman, E.F.; Mandel, J.S.; Morton, R.F.; Reding, D.J.; Rosenman, K.D.; Talaska, G., 2006: Gliomas and farm pesticide exposure in men: the upper midwest health study. The National Institute for Occupational Safety and Health evaluated farm pesticide exposure and glioma risk in a study that included 457 glioma cases and 648 population-based controls, all adult men (18-80 yr old) and nonmetropolitan residents of...

Robey-Cafferty, S.S.; Rutledge, M.L.; Bruner, J.M., 1990: Expression of a multidrug resistance gene in esophageal adenocarcinoma. Correlation with response to chemotherapy and comparison with gastric adenocarcinoma. The resistance of malignant tumors to chemotherapy is often associated with overexpression of the multidrug resistance gene MDR. Its gene product, P-glycoprotein, acts as a drug efflux pump for chemotherapeutic agents. The authors studied MDR expr...

Dilloo, D.; Zibert, A., 2003: Gene transfer for generation of tumor and leukemia vaccines. Methods in Molecular Biology 215: 261-277

Epstein M.A.F.; Ligas J.R.; Moslehi F.; Fletcher P.R.; Epstein R.A., 1986: Experimental determination of flows produced by piston pump high frequency oscillators. Federation Proceedings 45(4): 784

Zhang, Y.; Wu, J.; Jeevaratnam, K.; King, J.H.; Guzadhur, L.; Ren, X.; Grace, A.A.; Lei, M.; Huang, C.L-H.; Fraser, J.A., 2013: Conduction slowing contributes to spontaneous ventricular arrhythmias in intrinsically active murine RyR2-P2328S hearts. The familial condition catecholaminergic polymorphic ventricular tachycardia (CPVT) is characterized by episodic bidirectional ventricular tachycardia (BVT), polymorphic ventricular tachycardia (PVT), and ventricular fibrillation following adrener...

Eleftheriou, B. E.; Norman, R. L.; Summerfelt, R., 1968: Plasma levels of l,3,5, -estratiene-16-one. In the present study, levels of free plasma estradiol-17[beta], which was identified previously, and 16-ketoestradiol-17[beta] were made in the carp (Cyprinus carpio) with different ovarian weights and at different times before and after spawning.

Ounsi, H.F.; Salameh, Z.; Carvalho, C.Augusto.; Cantoro, A.; Grandini, S.; Ferrari, M., 2010: Bond strength of composite core build-up materials to fiber-reinforced posts: a microtensile comparison between conventional and wet-ethanol bonding systems. To evaluate the interfacial microtensile bond strength between a fiber-reinforced post (Rely X Post) and a core material (Multicore Flow) after placing different bonding agents. After post surface treatment, 50 posts were divided into 5 groups. Gr...

Hivernel F., 1983: Excavations at Ngenyn (Baringo District, Kenya). Azania 18: 45-79

Robertson, G. M., 1939: An Upper Silurian vertebrate horizon, with description of a new species, Cephalaspis oeselensis. Transactions of the Kansas Academy of Science, 42: 357-363