+ Translate

Spectra membrane binding and potentiometric responses of new charge shift probes

, : Spectra membrane binding and potentiometric responses of new charge shift probes. Biochemistry 24(21): 5749-5755

The properties of a series of new potentiometric membrane probes have been explored. The probes all contain an (aminostyryl)pyridinium chromophore or a more highly conjugated analogue. The spectral properties of the dyes are discussed in terms of the excitation-induced charge shift from the pyridine to the aniline; this charge shift also provides the basis for the voltage dependence of the spectra according to an electrochrome mechanism. The spectral responses to a membrane potential of a hemispherical bilayer have been obtained and, grossly, are quite similar for all probes tested. The more subtle variations from dye to dye can be partially rationalized by consideration of binding parameters, the depth within the membrane, and structural factors. The most potential sensitive dye in this collection has been designated di-4-ANEPPS and a 6-amino-2-naphthyl group in place of the p-anilino on the parent chromophore. Both the relative fluorescence emission and excitation responses have maxima of 8% per 100 mV, and these two spectra display a striking symmetry.

Accession: 006459340

PMID: 4084490

DOI: 10.1021/bi00342a010

Download PDF Full Text: Spectra membrane binding and potentiometric responses of new charge shift probes

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Simpson L.L.; Loew L.M.; Hassner A.; D'costa R., 1981: Emission response spectra for charge shift probes of membrane potential. Biophysical Journal 33(2 PART 2): 114A

Fluhler E.; Loew L.M., 1984: Membrane binding flip flop and voltage dependent spectroscopy of charge shift probes. Biophysical Journal 45(2 PART 2): 167A

Loew, L.M.; Simpson, L.L., 1981: Charge-shift probes of membrane potential: a probable electrochromic mechanism for p-aminostyrylpyridinium probes on a hemispherical lipid bilayer. The characteristics of the spectroscopic responses to membrane potential are examined for a series of dyes based on the 4-(p-aminostyryl)-1-pyridinium chromophore. An apparatus using an oxidized cholesterol hemispherical bilayer and phase-sensitiv...

Loew L.M.; Simpson L.; Hassner A.; Decosta R.; Parkins H., 1980: New charge shift probes of membrane potential. Federation Proceedings 39(6): ABSTRACT 2758

Hassner A.; Birnbaum D.; Loew L.M., 1984: Charge shift probes of membrane potential synthesis. Two general synthetic approaches are reported to a number of (aminostyryl)pyridinium dyes and their heterocyclic analogs which are of interest as electrochromic probes for membrane potential. The 2 routes, which involve palladium-catalyzed couplin...

Loew, L.M.; Bonneville, G.W.; Surow, J., 1978: Charge shift optical probes of membrane potential. Theory. The chromophores of a series of known and unknown probes of membrane potential are subjected to molecular orbital calculations. These calculations are used to characterize the charge distribution and excitation-induced shift of electron density in...

Loew, L.M.; Cohen, L.B.; Salzberg, B.M.; Obaid, A.L.; Bezanilla, F., 1985: Charge-shift probes of membrane potential. Characterization of aminostyrylpyridinium dyes on the squid giant axon. The characteristics of transmittance and fluorescence changes of 4-(p-aminostyryl)-1-pyridinium dyes in response to voltage-clamp pulses on the squid giant axon were examined. A zwitterionic styryl dye displays transmittance and excitation spectra...

Castanho, M.A.R.B.; Santos, N.C.; Loura, L.M.S., 1997: Separating the turbidity spectra of vesicles from the absorption spectra of membrane probes and other chromophores. The recording of the absorption spectra of membrane probes and other chromophores is frequently difficult because of turbidity. While in highly scattering media such as tissues, the solution to the problem can be rather complex, in cell and vesicl...

Witek, H.A.; Morokuma, K.; Stradomska, A., 2004: Modeling vibrational spectra using the self-consistent charge density-functional tight-binding method. I. Raman spectra. An extension of the self-consistent charge density-functional tight-binding (SCC-DFTB) method is presented that allows for calculating intensities of peaks in vibrational Raman spectra for very large molecules. The extension is based on a simple a...

Ehrenberg B.; Pevzner E., 1991: Absorption fluorescence and resonance raman spectroscopic probing of potentiometric membrane probes. Photochemistry & Photobiology 53(SUPPL): 106S