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Spectral analyses of fluorescences in dent maize infected with aspergillus flavus and aspergillus parasiticus



Spectral analyses of fluorescences in dent maize infected with aspergillus flavus and aspergillus parasiticus



Journal of Stored Products Research 12(4): 229-234



Bright greenish yellow (BGYF) and blue white (BWF) fluorescences were associated with A. flavus and A. parasiticus infected maize. The fluorescences were studied spectrofluorometrically, the BGYF exhibiting a peak wave length of 480-485 nm and the BWF 440-445 nm. Neither fluorescence varied in maize stored under different moistures and temperatures. BWF was similar spectrally to the fluorescence of the endosperm of sound kernels but 5-20 times more intense. The spectrum of BWF was similar to aflatoxin G1 or a mixture of aflatoxin B1, B2, G1 and G2 when they were spotted on endosperm tissue. A color reference for BGYF was similar in peak wave length to BGYF. 'Amsoy' soybeans without the seed coat fluoresced with a peak at 470-475 nm; the intensity was low compared to BGYF in maize. A fluorescence of maize kernels visually similar to BGYF but not associated with Aspergillus infection or aflatoxin contamination was also investigated. This false BGY fluorescence was spectrally similar to the BGYF in infected kernels.

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Accession: 006459372

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DOI: 10.1016/0022-474x(76)90038-2



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