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Spectral analysis of biochemical reactions used for identification of bacteria


, : Spectral analysis of biochemical reactions used for identification of bacteria. European Journal of Clinical Microbiology 3(4): 310-315

To define parameters for optimizing automated discrimination of bacterial biochemical reactions certain theoretical considerations of spectrophotometric analysis were explored. Recent clinical isolates (101) of gram-negative bacilli (21 spp.) were inoculated into AP1 20 E strips and read manually after 24 h. With spectrophotometric scanning the AP1 reactions could be classified inot 3 analytical categories: pH change, production of new products and darkening of the medium. Whereas single wavelength analysis gave 2.9% disagreement from the visual, multiple wavelength analyses were uniformly more accurate. The best results for pH change reactions were obtained by calculating a ratio of 2 wavelengths. New color reactions were best interpreted by demonstration of the new peak; darkening reactions required quantitation of the area under the entire curve. With these methods, a 99.3% overall agreement of individual reactions and a 97% agreement of identification were achieved. Multiple-point analysis of spectra coupled with computerized interpretation of the data should help resolve the problem of equivocal reactions in bacterial identification schemes optimized for spectral analysis.

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Accession: 006459385

PMID: 6386460

DOI: 10.1007/bf01977479

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Ramstedt U.; Serhan C.N.; Lundberg U.; Wigzell H.; Samuelsson B., 1984: Inhibition of human natural killer cell activity by 14 r 15 s 14 15 dihydroxy 5 z 8 z 10e eicosatetraenoic acid. Interactions between products of the 15-lipoxygenase cascade and human natural killer (NK) cell activity were studied. Addition of human leukocyte-derived (14R,15S)-14,15-dihydroxy-5Z,8Z,10E,12E-eicosatetraenoic acid (14,15-DiHETE) to the NK cytot...

Hashimoto, F.; Sugiura, K.; Inoue, K.; Ikehara, S., 1994: Enhancing effects of cyclosporin A on hematopoietic progenitors: possible role of CD8+ T cells as negative regulators. To elucidate the mechanism by which the administration of cyclosporin A (Cy A) improves a certain type of anemia, Cy A was orally administered to normal mice; hematopoietic activity, natural killer (NK) cell activity, and natural suppressor (NS) c...

Yuan, Y.; Granger, H. J.; Zawieja, D. C.; Mier, R. A.; Chilian, W. M., 1993: Histamine increases coronary venular permeability by phospholipase C-dependent synthesis of nitric oxide. FASEB Journal 7(3-4): A536

Quake, 1995: Fast Monte Carlo algorithms for knotted polymers. Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics 52(1): 1176-1180

Kondrat'ev, K. Ya; Bel'chanskii, G. I.; Petrosyan, V. G., 1992: Conceptual aspects of space monitoring of biodiversity. Reasons are described for the unsatisfactory state of the study of biodiversity. Several conceptual aspects of the space monitoring of biodiversity, which was not reflected in global programs of biosphere monitoring, are considered. Recommendation...

Klimczak, H.; Wiatkowska, B., 2006: The analysis of farm structure of villages Biadacz from the point of development of ecological agriculture. This study was conducted to analyse the structure of conventional farms in the village of Biadacz (Poland) and determine whether it favours conversion of farms in the whole village into ecological agriculture. Registration data based on land regis...

Vlasyuk, V. I., 1981: Yield and technological qualities of monogerm sugarbeet hybrids bred using male sterility and monogerm pollinators. Male-sterile monogerm forms were crossed with two progenies of different origin obtained by individual selection from male-fertile diploid material. Some hybrids exceeded the standard multigerm variety by 36.2-49.5 c/ha in yield, 0.6-0.8% in sugar...

Alkiewicz, J. A.; Jezowa, L.; Janiakowa, E., 1970: Occurrence of yeasts in the duodenum of children and unweaned infants. Samples taken from the duodenal contents of 245 patients from 3 months to 14 yr old who were suffering from inflammation of the gall bladder, pancreatitis, lambliosis and epigastric pains of unknown origin, yielded yeasts in 72 (29 %) cases. Candi...

Degrand Guillaud A.; Obadia Y.; Guelain J., 1991: Methodological survey on prevalence on alzheimers disease in the region of bouches du rhone. Revue d'Epidemiologie et de Sante Publique 39(4): 413-416

Kalueff, A. V.; Maisky, V. A.; Pilyavskii, A. I.; Makarchuk, N. E., 2001: Persistent c-fos expression and NADPH-d reactivity in the medulla and the lumbar spinal cord in rat with short-term peripheral anosmia. Here we examine hypothesis that short-term peripheral ZnSO4-induced anosmia can produce effects on c-fos expression within spinal cord and caudal medulla in male Wistar rats (n = 4). Fos-like-immunoreactive cells revealed by avidin-biotin-peroxida...