EurekaMag.com logo
+ Translate

Spectral analysis of cytochromes in aquaspirillum magnetotacticum


, : Spectral analysis of cytochromes in aquaspirillum magnetotacticum. Current Microbiology 15(3): 121-128

The respiratory chain of Aquaspirillum magnetotacticum strain MS-1 cells denitrifying microaerobically included a-, a1-, b-, c-, cd1-, and o-type hemes. More than 85% of the total cytochromes detected were of the c type. Virtually all of the a and b types were detected in cell membranes, whereas 70% of the c-type hemes were soluble. Large quantities of soluble c-type hemes were released with periplasm by freezing and thawing cells. Soluble c551 occurred in two forms: as a single compound of apparent molecular weight of 17,000 daltons, which bound CO, and, together withr d1 heme, as a component of nitrite reductase. Both a1-type hemes (which usually comprise part of the "low aeration" cytochrome oxidase) and o types (usually part of the "high aeration" oxidase) were simultaneously expressed in microaerobically grown denitrifying cells of A. magnetotacticum; this indicated branching of the respiratory chain.

(PDF 0-2 workdays service)

Accession: 006459398

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

O'brien W.; Paoletti L.; Blakemore R., 1984: Cytochromes in aquaspirillum magnetotacticum. Abstracts of the Annual Meeting of the American Society for Microbiology 84: ABSTRACT K45

Eden, P.A.; Schmidt, T.M.; Blakemore, R.P.; Pace, N.R., 1991: Phylogenetic analysis of Aquaspirillum magnetotacticum using polymerase chain reaction-amplified 16S rRNA-specific DNA. The 16S rRNA gene of the magnetotactic magnetogen Aquaspirillum magnetotacticum MS1 was amplified by a polymerase chain reaction, using two eubacterial consensus oligodeoxynucleotide primers flanking the majority of the 16S rRNA gene, cloned, and...

Eden P.A.; Schmidt T.M.; Blakemore R.P.; Pace N.R., 1991: Phylogenetic analysis of aquaspirillum magnetotacticum using polymerase chain reaction amplified 16s ribosomal rna specific dna. The 16S rRNA gene of the magnetotactic magnetogen Aquaspirillum magnetotacticum MS1 was amplified by a polymerase chain reaction, using two eubacterial consensus oligodeoxynucleotide primers flanking the majority of the 16S rRNA gene, cloned, and...

Schleifer K.H.; Schueler D.; Spring S.; Weizenegger M.; Amann R.; Lduwig W.; Koehler M., 1991: The genus magnetospirillum new genus description of magnetospirillum gryphiswaldense new species and transfer of aquaspirillum magnetotacticum to magnetospirillum magnetotacticum new combination. A magnetotactic spirillum (strain MSR-1) was isolated from the mud of the eutrophic river Ryck near Greifswald. Its morphological, ultrastructural and biochemical characteristics were compared with those of Aquaspirillum magnetotacticum. A cell su...

Paoletti, L.C.; Blakemore, R.P., 1986: Hydroxamate production by Aquaspirillum magnetotacticum. Spent culture fluids from Aquaspirillum magnetotacticum MS-1 grown at high (20 microM) but not low (5 microM) iron concentration contained material yielding a positive hydroxamate test. Cells possessed six major outer membrane proteins. Three oute...

Paoletti L.C.; Blakemore R.P., 1988: Iron reduction by aquaspirillum magnetotacticum. Iron reductase activity in cell extracts of Aquaspirillum magnetotacticum strain MS-1 (wild type) or nonmagnetotactic mutant strain NM-1A was located primarily in the periplasm. Cytoplasm contained 20%-35% and membrane fractions 3% of total iron r...

Bazylinski D.A.; Blakemore R.P., 1982: Nitrogen metabolism in aquaspirillum magnetotacticum. Abstracts of the Annual Meeting of the American Society for Microbiology 82: ABSTRACT I53

Short, K.A.; Blakemore, R.P., 1989: Periplasmic superoxide dismutases in Aquaspirillum magnetotacticum. Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was loc...

Maratea D.; Blakemore R.P., 1981: Aquaspirillum magnetotacticum new species a magnetic spirillum. The taxonomically relevant characteristics of a microaerophilic, chemoheterotrophic, magnetotactic, freshwater spirillum were studied. This organism represents a new bacterial species, which is most appropriately placed in Aquaspirillum despite it...

Bazylinski, D.A.; Blakemore, R.P., 1983: Denitrification and Assimilatory Nitrate Reduction in Aquaspirillum magnetotacticum. Aquaspirillum magnetotacticum MS-1 grew microaerobically but not anaerobically with NO(3) or NH(4) as the sole nitrogen source. Nevertheless, cell yields varied directly with NO(3) concentration under microaerobic conditions. Products of NO(3) red...