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Spectral analysis on the melting fine structure of phage lambda dna and phage t 2 dna



Spectral analysis on the melting fine structure of phage lambda dna and phage t 2 dna



Biopolymers 16(2): 427-435



The fine structure of the thermal denaturation of viral DNA was analyzed with a previously established spectral method. A fully automated system measuring melting curves at 4 wavelengths at the same time was developed for this purpose. With this device, (G + C)-contents of melting components of .lambda. DNA were determined. The correlation between the (G + C)-content and the melting temperature of each melting component is well described by the Marmur and Doty linear relationship. The denaturation of each component is regarded as cooperative. The Marmur-Doty relation also stands for the local denaturation of T2 DNA, which has a narrow melting range.

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