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Spectral and functional characterization of membrane fragments from the facultative photosynthetic bacterium rhodopseudomonas blastica


Archives of Microbiology 140(1): 15-20
Spectral and functional characterization of membrane fragments from the facultative photosynthetic bacterium rhodopseudomonas blastica
The cytochromes of photosynthetically grown R. blastica were thermodynamically characterized using the technique of redox titrations. Six cytochromes were present: 2 cytochromes c, Em7 = + 295 mV, Em7 = +345 mV; and 4 cytochromes b, Em7 = +290 mV, Em7 = +130 mV, Em7 = +60 mV, Em7 = -4mV. these cytochromes were tightly bound except for cytochrome c with Em7 of +345 mV which was mostly present in the soluble cell extracts. The effects of cyanide on both the cytochrome c oxidase activity and the NADH-dependent respiration revealed the presence of a branched respiratory chain, 1 branch leading to a cyanide-resistant oxidase containing pathway and the other including the cyanide-sensitive cytochrome c-oxidase. The effects of antimycin A, myxothiazole and 5-undecyl-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) on the steady-state NADH-dependent respiration were also studied. Antimycin A and myxothiazole appeared to act at the level of the umbiquinol-cytochrome c oxidoreductase, while UHDBT drastically affected both respiratory branches. Absorption spectra of chromatophore photopigments resulted to be similar to those reported in many species of facultative photosynthetic bacteria although carotenoid absorption maxima were blue-shifted by 5 nm. The light-induced O2 reduction performed by chormatophores from R. blastica suggested a strict interaction between photosynthetic and respiratory apparatus.

Accession: 006459494

DOI: 10.1007/bf00409765

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