EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectral changes in cytochrome p 450 of rat liver microsomes during its interaction with phenazepam and 3 hydroxy metabolite






Byulleten' Eksperimental'noi Biologii i Meditsiny 97(8): 196-198

Spectral changes in cytochrome p 450 of rat liver microsomes during its interaction with phenazepam and 3 hydroxy metabolite

Phenazepam (1) and 3-hydroxymetabolite (II) interacting with cytochrome P-450 in rat liver administered phenobarbital and 3-methylcholantrene demonstrated the 2nd type of spectral changes in hemoprotein. The binding constants of I and II differed considerably which points to the possibility of interaction of substrates with different areas of hemoproteins. The characteristic points (maximum, minimum, isobestic) of the cytochrome P-450 spectrum were displaced during II titration to the longwave region as compared with I. The calculation of electron density on the atoms of the heterocycle and aromatic nuclei of the molecules of I and II showed that the differences in the spectra of I and II binding with cytochrome P-450 are determined by the distribution of electron density of their heterocycle.

(PDF 0-2 workdays service: $29.90)

Accession: 006459543



Related references

Spectral changes in the liver microsomal cytochrome P-450 of rats during interaction with phenazepam and its 3-hydroxy metabolite. Biulleten' Eksperimental'noi Biologii i Meditsiny 98(8): 196-198, 1984

Spectral interaction of propranolol with cytochrome p 450 of rat liver microsomes. Federation Proceedings 36(3): 1031, 1977

Method of quantitative gas chromatographic determination of phenazepam and its metabolite 3 hydroxy phenazepam in plasma. Pharmaceutical Chemistry Journal (English Translation of Khimiko-Farmatsevticheskii Zhurnal) 14(8): 579-582, 1980

Model of the kinetics of blood levels of phenazepam and the metabolite 3 hydroxy phenazepam in cats. Bulletin of Experimental Biology & Medicine 94(10): 1373-1375, 1982

Method of quantitative gas chromatographic determination of phenazepam and its metabolite 3 hydroxy phenazepam in human plasma. Khimiko-Farmatsevticheskii Zhurnal 14(8): 97-100, 1980

Optical spectral studies of ebselen interaction with cytochrome P-450 of rat liver microsomes. Biochemical Pharmacology 38(4): 619-625, 1989

Modeling the kinetics of phenazepam and its metabolite 3 hydroxy phenazepam in cat blood. Byulleten' Eksperimental'noi Biologii i Meditsiny 94(10): 54-56, 1982

Spectral interaction of 11-hydroxy-delta-8-tetrahydrocannabinol with rabbit and rat liver microsomal cytochrome P-450. Chemical & Pharmaceutical Bulletin 28(6): 1939-1942, 1980

Ellipticines and human liver microsomes: spectral interaction with cytochrome P-450 and hydroxylation. Inhibition of aryl hydrocarbon metabolism and mutagenicity. Chemico-Biological Interactions 36(3): 299-309, 1981

Metabolism of rifabutin and its 25-desacetyl metabolite, LM565, by human liver microsomes and recombinant human cytochrome P-450 3A4: relevance to clinical interaction with fluconazole. Antimicrobial Agents and ChemoTherapy 41(5): 924-926, 1997