+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectral changes in fluorescence of phycobilisomes in the course of dissociation

Photosynthetica (Prague) 18(2): 179-183
Spectral changes in fluorescence of phycobilisomes in the course of dissociation
Spectral changes in fluorescence of phycobilisomes (PBS) of Anabaena variabilis in 0.9, 0.6, 0.3 and 0.1 M phosphate buffer at different dissociation times were studied at room or liquid N temperature. The room temperature fluorescence emission maximum of intact PBS was at 678 nm, that of completely dissociated PBS at 644 nm. There were 3 bands in the liquid N temperature fluorescence emission spectrum of PBS: F655, F666 and F686, characteristic of C-phycocyanin (C-PC), allophycocyanin (APC) and APC-B, respectively. The relative fluorescence of F686 in intact PBS was very high, and those of F655 and F666 were low. In the course of PBS dissociation, the relative fluorescence of F686 decreased, and those of F655 and F666 increased. In completely dissociated PBS, the relative fluorescences of F655 and F666 were very high, and F686 disappeared. The linkage between APC and APC-B apparently is unstable while that between C-PC and APC is comparatively stable.

Accession: 006459544

Related references

Spectral changes in fluorescence of phycobilisomes under conditions of denaturing. Soviet plant physiology (pub 1981) 27(1): 570-577, 1981

Cyanobacterial phycobilisomes: Selective dissociation monitored by fluorescence and circular dichroism. Proceedings of the National Academy of Sciences of the United States of America 77(4): 1961-1965, 1980

Studies on the fluorescence emission spectra and energy transfer of phycobilisomes from Spirulina platensis in the course of dissociation. Murata, N [Editor] Research in photosynthesis, Vol 1; IXth International Congress on Photosynthesis : 61-64, 1992

Fluorescence emission, energy transfer and structure of phycobilisomes from Nostoc flagelliforme Born. et Flah. during dissociation. Photosynthetica (Prague) 28(2): 253-258, 1993

Dissociation of phycobilisomes in representatives of different division algae. Ukrayins'kyi Botanichnyi Zhurnal 51(6): 102-108, 1994

Dissociation kinetics of anabaena variabilis phycobilisomes. Plant & Cell Physiology 23(5): 803-812, 1982

Dissociation and reconstitution of cyanophyte membrane bound phycobilisomes. Israel Journal Of Medical Sciences: 489, 1981

The Calibration Kit Spectral Fluorescence Standards--a simple and certified tool for the standardization of the spectral characteristics of fluorescence instruments. Journal of Fluorescence 16(4): 581-587, 2006

Spectral analysis of allophycocyanin I, II, III and B from Nostoc sp. phycobilisomes. Biochimica et Biophysica Acta 592(2): 267-276, 1980

Dissociation of isolated cyanobacteria phycobilisomes after crosslinking with diimidoesters and glutaraldehyde Algae. Photosynthetic prokaryotes: cell differentiation and function: proceedings of the Special FEBS Meeting April 25-29-1982 Athens Greece editors GC Papageorgiou L Packer: 7, 1983