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Spectral changes in membrane fragments and artificial liposomes of anacystis nidulans induced by chilling


, : Spectral changes in membrane fragments and artificial liposomes of anacystis nidulans induced by chilling. Archives of Biochemistry & Biophysics 193(2): 385-391

Isolated membrane fragments from A. nidulans grown at 39.degree. C undergo visible spectral changes on chilling, suggesting a carotenoid component is altered. No such changes are seen when cells are grown at 25.degree. C. The magnitude of the decreased absorbance is a function of the chilling temperature and the media in which membrane fragments are suspended. The spectral decrease following chilling develops relatively slowly and is a function of the cooling rate and final temperature. The absorbance change is reversed if the fragments are heated to near 50.degree. C subsequent to chilling. Liposomes prepared from a total lipid extract of Anacystis undergo a spectral change on chilling which closely resembles that occurring in whole cells or isolated membrane fragments. Liposomes prepared from an extract of cells grown at 25.degree. C show only about 30% as great a spectral change as those from cells grown at 39.degree. C. The spectral bleaching is freely reversible when the liposomes are reheated, but shows a pronounced hysteresis. Apparently specific phase changes occur in Anacystis membranes and artificial liposomes on cooling which alter the environment of carotenoid. These changes may relate to previous observations that cells grown at 39.degree. C cannot survive a cold shock while those grown at 25.degree. C do.

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Related references

Brand, J.J., 1979: Spectral changes in membrane fragments and artificial liposomes of Anacystis induced by chilling. Isolated membrane fragments from Anacystis nidulans grown at 39 °C undergo visible spectral changes on chilling, suggesting a carotenoid component is altered. No such changes are seen when cells are grown at 25 °C. The magnitude of the decreased...

Brand, J.J., 1977: Spectral Changes in Anacystis nidulans Induced by Chilling. When A. nidulans strain TX 20 was grown at 39.degree. C then rapidly chilled to O.degree. C, a pigment with a carotenoid-like spectrum was bleached. This effect was not seen when cells which were grown at 25.degree. C were chilled. The effect seen...

Ono, T.A.; Murata, N., 1981: Chilling susceptibility of the blue green alga anacystis nidulans 2. stimulation of the passive permeability of cytoplasmic membrane at chilling temperatures. K ions and amino acids were found to leak from the cytoplasm to the outer medium when the blue-green alga, A. nidulans, was exposed to the chilling temperatures. The leakage was marked below the critical temperature regions, the midpoint values fo...

Ono, T.A.; Murata, N., 1981: Chilling Susceptibility of the Blue-green Alga Anacystis nidulans: II. STIMULATION OF THE PASSIVE PERMEABILITY OF CYTOPLASMIC MEMBRANE AT CHILLING TEMPERATURES. Potassium ions and amino acids were found to leak from the cytoplasm to the outer medium when the blue-green alga, Anacystis nidulans, was exposed to the chilling temperatures. The leakage was marked below the critical temperature regions, the mid...

Murata N.; Ono T A.; Sato N., 1979: Lipid phase of membrane and chilling injury in the blue green alga anacystis nidulans. Lyons, J M , D Graham And J K Raison (Ed ) Low Temperature Stress in Crop Plants: The Role Of The Membrane; Proceedings Of An International Seminar, Honolulu, Hawaii, Usa, Mar 26-30, Xv+565p Academic Press, Inc : New York, N Y , Usa; London, England Illus P337-346

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