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Spectral characteristics of acridine orange fluorochromed human white blood cells/

, : Spectral characteristics of acridine orange fluorochromed human white blood cells/. Biofizika 22(6): 1015-1023

The ratio (.alpha. = I640/I530) of the luminescence intensity of the red spectrum region (640 nm) to the green spectrum region (530 nm) of acridine orange fluorochromed cells fixed under certain conditions is a specific character. The latter can be used for automatic classification of bone marrow cells and peripheral blood and for the diagnosis of some pathological states of the cell. The type of changes of the ratio at photochemical bleaching of fluorochromed cells under irradiation (436 nm) depends on the level of cell differentiation. Completely differentiated cells are characterized by a simultaneous decrease of luminescence intensity in the red (640 nm) and green (530 nm) spectrum regions. In undifferentiated cells (especially at the blast stage) a decrease of luminescence intensity in the red region (640 nm) is accompanied by an increase of the luminescence intensity in the green region (530 nm). The effect of photobleaching of undifferentiated cells may be due to the photodestruction of dimers of acridine orange bound with monohelical regions of DNA.

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