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Spectral characteristics of allo phycocyanin isolated from anabaena cylindrica curve fitting analysis


, : Spectral characteristics of allo phycocyanin isolated from anabaena cylindrica curve fitting analysis. Archives of Biochemistry & Biophysics 215(1): 266-273

Spectroscopic characteristics of allophycocyanin (APC) isolated from A. cylindrica were studied by curve-fitting analyses of absorption spectra measured at room temperature. Gaussian curve-fittings based on the least-squares method were done on absorption spectra for 3 different dissociation-association conditions of the protein. The composition of the component bands was similar in the 3 absorption spectra and could be classified into 3 groups depending on changes in band intensity; the 1st group was represented by the main band at 653 nm, which was intense in the trimer APC but markedly reduced in the monomer. The 2nd group was represented by the main band at 607 nm, which showed opposite changes. The 3rd group had its main band at 623 nm, and it remained the same in the 3 absorption spectra. The sum of the band area of the variable components (the 1st and 2nd groups) was almost equal to that of the constant components (the 3rd group). APC has 2 types of phycocyanobilin (PCB) which differ in their spectroscopic characteristics. The PCB responsible for the variable component bands, and thus for the spectral changes, has 2 excitation states corresponding to 653 and 607 nm, respectively, and the spectral changes are ascribable to variations in the relative frequencies of occurrence of the 2 states; in associated APC, the 1st state predominates, and, in dissociated APC, the 2nd. PCB for the constant component bands has only 1 excitation state corresponding to the 623-nm band. Changes in the emission spectra correlate with these changes in the absorption spectra, suggesting that in APC the phycocyanobilins responsible for and independent of absorption spectrum changes act as the fluorescing and sensitizing types, respectively.

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