EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectral characteristics of asparaginyl and valyl transfer rna synthetases from normal and long fasting rabbit muscles


Molekulyarnaya Biologiya (Moscow) 18(5): 1330-1335
Spectral characteristics of asparaginyl and valyl transfer rna synthetases from normal and long fasting rabbit muscles
Conformational differences between asparagyl- and valyl-tRNA synthetases from normal (ARS.eta.) and long-fasting (ARS.intg.) rabbit's muscle were revealed by means of UV fluorescence and differential spectroscopy. The fluorescence spectra indicate more hydrophobic environment of tryptophan residues in the ARS.intg. at similar quantum yields. The differential absorption spectra reveal the distinctions between tryptophanyl microenvironments for ARS's of different amino acid specificity and for ARS.eta. and ARS.intg. of the same specificity. Lower accessibility of tryptophan residues in ARS.eta. in comparison with ARS.intg. was shown by selective fluorescence quenching with Cs+ and I- ions. The effect of longwave shift of fluorescence spectra at the edge excitation was used for studies on microenvironment of chromophors and the rate of the dipole-orientational relaxation of this microenvironment.


Accession: 006459561



Related references

Isolation and purification of aspartyl and valyl transfer rna synthetases from normal and long fasting rabbit muscles their structural peculiarities and some kinetic parameters. Molekulyarnaya Biologiya (Moscow) 18(5): 1369-1375, 1984

Isolation and purification of aspartyl- and valyl-tRNA-synthetases from muscles of normal and long-fasting rabbits. Structural characteristics and various kinetic parameters. Molekuliarnaia Biologiia 18(5): 1369-1375, 1984

Spectral characteristics of muscular aspartyl transfer rna synthetases and valyl transfer rna synthetases and their complexes with substrates in norm and after long term starvation. Ukrainskii Biokhimicheskii Zhurnal 56(5): 519-526, 1984

Purification and properties of aspartyl transfer rna synthetase and valyl transfer rna synthetase from muscles of normal and long fasting rabbits. Doklady Akademii Nauk Ukrainskoi SSR Seriya B Geologicheskie Khimicheskie i Biologicheskie Nauki (9): 63-67, 1983

Limited proteolysis of aspartyl transfer rna synthetase and valyl transfer rna synthetase from muscles of normal and long fasting rabbits. Doklady Akademii Nauk Ukrainskoi SSR Seriya B Geologicheskie Khimicheskie i Biologicheskie Nauki (12): 55-58, 1984

Characteristics of the structure and specificity of aspartyl- and valyl-tRNA-synthetases from muscle tissue of long-fasting rabbits. Ukrainskii Biokhimicheskii Zhurnal 59(5): 32-36, 1987

Spectral characteristics of muscle aspartyl- and valyl-tRNA- synthetases and their complexes with substrates in normal conditions and after prolonged starvation. Ukrainskii Biokhimicheskii Zhurnal 56(5): 519-526, 1984

Spectral characteristics of muscle aspartyl- and valyl-tRNA- synthetases in normal animals and in an experimental model of prolonged starvation. Molekuliarnaia Biologiia 18(5): 1330-1335, 1984

Fidelity in the aminoacylation of valyl transfer rna with hydroxy analogues of valine leucine and isoleucine by valyl transfer rna synthetases from saccharomyces cerevisiae and escherichia coli. Biochemistry 29(34): 7953-7958, 1990

The poly amine specificity of the valyl transfer rna synthesis reaction catalyzed by prokaryotic escherichia coli and eukaryotic plant lupinus luteus valyl transfer rna synthetases. Bachrach, Uaye And R. Chayen (ed.). Advances In Polyamine Research, Vol. 4. 41st Bat-Sheva Seminar On Polyamines In Growth And Differentiation Processes, Kiryat Anavim, Jerusalem, Israel, July 4-9, . Xxiv 808p. Raven Press: New York, N.y., Usa. Illus. P307-320, 1983