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Spectral characterization of cytochrome p 450 of a strain candida tropicalis grown on tetra decane


, : Spectral characterization of cytochrome p 450 of a strain candida tropicalis grown on tetra decane. European Journal of Biochemistry 109(1): 103-108

Several properties of the cytochrome P-450 induced in the yeast C. tropicalis by growth on tetradecane were studied by differential visible spectroscopy on microsomes. The spectral changes typical of this cytochrome were obtained by subtraction of an unspecific spectral change, possibly due to the presence of other hemoproteins in microsomes, from the experimental difference spectra. Like the previously described cytochromes P-450 from yeast [Saccharomyces cerevisiae] and mammalian liver, C. tropicalis cytochrome P-450 is in spin-state equilibrium at ambient temperature: about 30% of the originally low-spin cytochrome is converted to the high-spin state upon increasing the ionic strength of the medium, while 30% of the originally high-spin cytochrome is converted to the low-spin state upon addition of hydrophobic alcohols. C. tropicalis cytochrome P-450 readily binds nitrogenous ligands, isocyanides and phosphines in the ferric and ferrous state with spectral characteristics similar to those reported for other yeast or mammalian cytochromes P-450. It also reacts successively with cumylhydroperoxide and 1,3-benzodioxole to form a high-valent iron-oxo species and an iron-carbene metabolite complex. However, it fails to produce any spectral or spin-state change upon addition of hydrophobic non-coordinating compounds such as n-tetradecane, its substrate in vivo.

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