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Spectral dimorphism of crayfish procambarus clarkii visual pigment in solution

, : Spectral dimorphism of crayfish procambarus clarkii visual pigment in solution. Vision Research 22(7): 727-738

Crayfish (Procambarus) photoreceptor membranes were prepared by a new technique. Digitonin extracts made at contain 2 rhodopsin-like pigments, P562 and P512., in a ratio of .apprx. 5:4. On exposure to light at C, P562 is converted to a metarhodopsin-like photoproduct with .lambda.max at 515 nm (M515), and P512 is converted to a metarhodopsin-like photoproduct with .lambda.max at 475 nm (M475). When warmed to C, both photoproducts bleach in minutes to retinal and opsin. Neither P562 nor P512 is attacked by hydroxylamine, but both are destroyed by the detergent Ammonyx LO and by reduction of disulfide bridges with 2-mercaptoethanol. Neither is altered by changing the concentration of Cl-. P562 is more susceptible than P512 to thermal denaturation and attack by OH-, and its spectrum is distinctly narrower than predicted by an Ebrey-Honig nomogram. P562 and P512 do not interconvert, either in the dark or in the presence of light. P512 is distinct from M515; it is substantially more stable at C and has a 23% smaller molar extinction coefficient. M515 is spectrally similar to the single metarhodopsin that is formed by irradiation of rhabdoms from dark-adapted crayfish. As neither P562 nor P512 is found in crayfish rhabdoms. It is concluded that both pigments are created in digitonin micelles from a spectrally and kinetically homogeneous population of rhodopsin molecules with .lambda.max near 530 nm in the rhabdom.

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