+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectral effects of protochlorophyllide aggregation

Molekulyarnaya Biologiya (Moscow) 19(4): 915-925
Spectral effects of protochlorophyllide aggregation
Protochlorophyllide isolated from etiolated leaves through self-assembly forms in solid films aggregated structures with main absorption maxima at 630 (PChd630) and 638 nm (PChd638), and fluorescence maxima at 633 and 640 nm, respectively. In the ammonia vapour each form is rearranged into the structure absorbing at 655 nm and with the fluorescence maximum of about 658 nm. In the presence of ammonium hydroxide the self-assembly of PChd655 was also observed in the aqueous solution of the pigment. In all the systems pChd655 is characterized by thermolability. The heating up to C causes its transformation into PChd630 and PChd638. The oxidation rate for PChd655 is faster in comparison to shortwave forms. PChd655 self-assembly as shown by measurements of solid films occurs through the formation of two types of the intermolecular bonds, which involve ketogroups of cyclopentanone rings keto C.dbd.0..HN(R)..Mg) and carboxylgroups of the pyrrole ring IV (propyl C.dbd.0..HN(R)..Mg). During the self-assembly if shortwave forms carbonyl groups of the molecules are not involved in the intermolecular interaction. The study on CD-spectra leads to the conclusion that PChd655, unlike PChd 630 and PChd 638, has the regular structure, which may consist of dimers with approximately parallel mutual molecular arrangement. The protochlorophyllide forms studied model the forms of the chlorophyll (chlorophyllide) precursor in plants. Peculiarities of the protochlorophyllide intermolecular interaction and the state of the chlorophyll (chlorophyllide) precusor in plants are discussed.

Accession: 006459656

Related references

Protochlorophyllide aggregation in solution and associated spectral changes. Biochimica et Biophysica Acta 153(3): 685-691, 1968

Protochlorophyllide and POR development in dark-grown plants with different proportions of short-wave length and long-wavelength protochlorophyllide spectral forms. Physiologia Plantarum 128(4): 751-762, 2000

Protochlorophyllide and POR development in dark-grown plants with different proportions of short-wavelength and long-wavelength protochlorophyllide spectral forms. Physiologia plantarum 128(4): 751-762, 2006

Fluorescence lifetimes of protochlorophyllide in plants with different proportions of short-wavelength and long-wavelength protochlorophyllide spectral forms. Photochemistry and Photobiology 78(2): 205-212, 2003

Effects of high concentrations of 5-aminolevulinic acid on plastid structure and spectral characteristics of protochlorophyllide in barley leaves. Fiziologiya Rastenii (Moscow) 41(3): 404-408, 1994

A 1H NMR spectroscopy of protochlorophyllide Mg. Photoreduction of protochlorophyllide Mg and protochlorophyllide Zn in etioplast lamellae. Advances in photosynthesis research Vol IV: 753-756, 1984

Protochlorophyllide spectral forms. Pakistan Journal of Biological Sciences 13(12): 563-576, 2011

Identification of spectral forms of protochlorophyllide in the region 670-730 nm. Photochemical & Photobiological Sciences 4(2): 230-238, 2005

Aggregation of monovinyl- and divinyl-protochlorophyllide in organic solvents. Photochemistry and photobiology 52(1): 95-101, 1990

Spectral properties of cytochrome protochlorophyllide-450 fr om bacteroids of lupine nodules. Doklady Akademii nauk SSSR: 16 (5) 1185-1187, 1974