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Spectral evidence for 2 forms of acid ferri hemo globin

Archives of Biochemistry and Biophysics 175(1): 138-143

Spectral evidence for 2 forms of acid ferri hemo globin

The spectral changes brought about by the use of glycerol-water mixtures as a solvent for ferrihemoglobin [human] were studied. In the presence of substantial amounts of glycerol a form of acid ferrihemoglobin spectrally resembling that of the imidazole complex of ferrihemoglobin was stabilized. A quantitative analysis of the spectral changes suggested that the effect of glycerol on ferrihemoglobin may in large part be ascribed to its effect on the activity of the solvent water.

Accession: 006459673

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