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Spectral evidence for 3 metal linked ionization equilibria in the interaction of cobalt ii horse liver alcohol dehydrogenase ec 1.1.1.1 with coenzyme and substrate


, : Spectral evidence for 3 metal linked ionization equilibria in the interaction of cobalt ii horse liver alcohol dehydrogenase ec 1.1.1.1 with coenzyme and substrate. Journal of Inorganic Biochemistry 17(3): 227-236

The visible absorption bands in the region 525-575 nm of the catalytic Co ion in Co(II) horse liver alcohol dehydrogenase show characteristic pH-dependent changes in the free enzyme and its complexes with NAD+ and NAD+ plus ethanol or 2,2,2-trifluoroethanol. In the free enzyme, the change of the coordination environment has an apparent pK of .apprx. 9.4. In the binary complex with NAD+ the spectral changes are complex, indicating changes in the coordination sphere in a lower pH range with an estimated pK value of .apprx. 7.9. The ternary complexes enzyme.cntdot.NAD+.cntdot.ethanol and enzyme.cntdot.NAD+.cntdot.2,2,2-trifluoroethanol exhibit very similar, characteristic spectral features; their apparent pK values are 6.3 and < 4, respectively. These pK values are ascribed to the ionization of the alcohol bound in the ternary complexes. The catalytic Co senses changes in the ionization state of the protein when going from low pH forms to high pH forms in the absence and presence of coenzyme and substrate/inhibitor.

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Mathur P. C., 1955: Zircon in Bundelkhand granites and associated rocks. Reports data on crystal habit, size variation, and inclusions in zircons from the Hamirpur district, India.

Mills Jr.; G. P.; Fales, J. H.; Durbin Jr.; C. G., 1969: Comparison of the effect of six pyrethroids against a backswimmer, Notonecta undulata Say. In laboratory tests in Maryland, six pyrethroids were compared with difenphos (Abate) for toxicity to adults of Notonecta undulata Say collected in the field and exposed to the compounds in distilled water. At 0.02 part per million (the highest co...

Pruszynski, S.; Rosada, J., 1987: Researches on the migration and hibernation of predatory bug Podisus maculiventris Say using radiotracer 32P. The migration and overwintering of Podisus maculiventris, an introduced predator of the potato pest Leptinotarsa decemlineata, were studied in Poland in 1980-84 using a radioactive tracer technique. Breeding and polyphagy in potato fields were est...

Pearson David V., 1966: Study of a slide, south of Rapid City, South Dakota. Proceedings of the South Dakota Academy of Science 45: 287

Nuti, K. M.; Meyer, R. K., 1975: Effects of partial lutectomy in pregnant mare serum gonadotropin primed immature pregnant rats. Biology of Reproduction 13(4): 415-422

Grochalska, D.; Mroczek, J., 2001: Influence of soyabean preparations and reduced salt content on the quality of poultry sausages. The possibility of reducing salt content from 2.5 to 1.5% in homogenized poultry sausages using soya protein concentrates or isolates was studied.

Ross, S. A.; Desai, H. K.; Joshi, B. S.; Srivastava, S. K.; Glinski, J. A.; Si, Y. C.; Pelletier, S. W., 1988: The structure and partial synthesis of delelatine, an alkaloid from Delphinium species. The roots of D. tatsienense, native to Yunnan, China are used in Chinese medicine. A new C-19 diterpenoid alkaloid designated as delelatine was isolated from D. elatum and D. tatsienense and its structure was elucidated by spectroanalytical method...

Harbeck, R. J.; Hoffman, A. A.; Hoffman, S. A.; Shucard, D. W.; Carr, R. I., 1978: A naturally occurring antibody in new zealand mice cyto toxic to dissociated cerebellar cells. A spontaneously occurring antibody cytotoxic to dissociated cells of the neonate mouse cerebellum was found in the sera of some New Zealand (NZ) mice. No significant activity was found in the sera of 6 non-autoimmune mouse strains. The degree of c...

Shalimov S. L., 2006: On effect of precession-induced flows in the liquid core for early Earth's history. Secondary and tertiary flow patterns seen in experiments simulating flow in the Earth's liquid core induced by luni-solar precession of the solid mantle (Vanyo et al., 1995) hint at the development of non-axisymmetric columnar periodic struct...

Fajmonova, E., 1976: Forest communities of the Javorniky mountains and the northern part of the Biele Carpathian mountains. Acta Botanica: 4 101-138