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Spectral evidence for distinct mode of interaction of nucleotides with rabbit muscle and rabbit liver aldolase ec 4.1.2.13


, : Spectral evidence for distinct mode of interaction of nucleotides with rabbit muscle and rabbit liver aldolase ec 4.1.2.13. Biochimica et Biophysica Acta 667(1): 218-222

UV difference spectra produced by the binding of mononucleotides and phosphates to rabbit aldolase A and B were analyzed. Both isozymes exhibit a distinct mode of interaction with the ligands. The binding seems to be based on multipoint interaction of nucleotides with each aldolase, indicating the existence of specific nucleotide binding domains in both proteins.

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