+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectral evidence for weak ligand in 6th position of hepatic microsomal cytochrome p 450 low spin ferric iron in vivo

, : Spectral evidence for weak ligand in 6th position of hepatic microsomal cytochrome p 450 low spin ferric iron in vivo. Pharmacology (Basel) 17(5): 262-279

The spin state of cytochrome P-450 ferric iron in vivo and in vitro was examined in freshly prepared liver microsomes from rabbit, guinea pig, hamster, mouse and rat by room-temperature difference spectrophotometry. The administration of compounds which induce certain forms of P-450 (phenobarbital, .beta.-naphthoflavone or 3-methylcholanthrene) to each of these species produces consistent detectable changes in the spin state of P-450 iron in vivo. Simple liquids of known relatively pure type I, reverse type I or type II character (cyclohexane, butanol-1 and octylamine-1, respectively) produce detectable changes in the in vitro spin state of P-450 iron. By the method of off-balance spectrophotometry (in which microsomal cytochrome b5 is made equal in both cuvettes), these differences in spin state in vivo and in vitro were shown to vary among the 5 mammalian species and are altered by prior treatment in vivo with the inducers of P-450 and by addition in vitro of such chemicals as cyclohexane, butanol-1 and octylamine-1. The magnitude of the peak-to-trough height in type I, reverse type I and type II difference spectra (or whether any spectrum is detected) is shown to depend largely on the in vivo spin state of the P-450 iron in freshly prepared microsomes at the start of the experiment. The presence of a 410 nm trough in the type II difference spectrum represents a ligand having reverse type I character in the 6th coordination site of in vivo low spin iron. Apparently the 6th ligand for in vivo low spin P-450 ferric iron is a hydroxyl group from an adjacent amino acid residue of the P-450 protein (or moiety of similar ligand field strength, e.g., a water model molecule) rather than a stronger ligand such as one having lone pair electrons from an N atom.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006459684

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Kartusch, B., 1975: Simultaneous demonstration of separate phases with different phenol content in the leaf epidermis cells of Salix alba -L. Protoplasma 6 (4) 371-379

Kubota, K.; Furumoto, S.; Iwata, R.; Ido, T.; Hara, T.; Fukuda, H., 2003: Autoradiographic comparison of intra-tumoral distribution of 18F-fluoromethyl choline, 2DG, methionine, and choline. Journal of Nuclear Medicine 44(5 Supplement): 82P, May

Tian ZhiHong; Meng JinLing, 1996: Advances in research on the molecular biology of male sterility in oilseed rape. This review of the molecular biology of male sterility in oilseed rape discusses the structure and function of the cytoplasmic genetic system in the mitochondria and the chloroplasts. The nature of the genetic material in the mitochondria, chlorop...

Roberts, G.; Cohen, J.; Khan, N.; Irava, W., 2010: Attempted suicide in Western Viti Levu, Fiji. Case records were reviewed of 132 people referred to the FSEG in Lautoka from January 2004 to December 2005 following an attempt at suicide. Seventy five percent of the study group was under age 32, 90% were Indo-Fijian and 66% female; these chara...

Svetovidov, A. N., 1959: Systematical situation of Trachurus la-certa Problems in ichthyology. In 1811 Pallas described 2 spp. of scads from the Black Sea: Scomber trachurus (= Trachurus mediterraneus ponticus) and S. lacerta. The taxonomy of S. lacerta was not clear for a long time. An unpublished drawing by Pallas, preserved in the archiv...

Bom, I.; van Wassenaar, D.; Boot, J., 1998: Hybrid affinity chromatography of alpha-galactosidase from Verbascum thapsus L. Purification of alpha-galactosidase from the roots of Verbascum thapsus L. was difficult to achieve using conventional methods due to the presence of coloured contaminants. A newly developed procedure, hybrid affinity chromatography, which was bas...

Kurepina, M., 1926: Entwicklung der primaren Choanen bei Amphibia. 1. Anura Rev Zool Russe Moscow, 63: 53-72

Huang, S-shiung.; Li, I-Hsun.; Hong, P-da.; Yeh, M-kung., 2014: Evaluation of protective efficacy using a nonstructural protein NS1 in DNA vaccine-loaded microspheres against dengue 2 virus. Dengue virus results in dengue fever or severe dengue hemorrhagic fever/dengue shock syndrome in humans. The purpose of this work was to develop an effective antidengue virus delivery system, by designing poly (dl-lactic-co-glycolic) acid/polyethy...

HOSEIN.E.A.; PROULX.P.; ARA.R., 1962: Substances with acetylcholine activity in normal rat brain. Extracts of normal rat brain which are assumed to contain the "total acetylcholine" content of the tissue were treated with Reinecke salt at alkaline pH to precipitate quaternary amines and other nitrogen compounds. Chemical examination...

Ravikumar, S.; Inbaneson, S.Jacob.; Suganthi, P.; Gnanadesigan, M., 2011: In vitro antiplasmodial activity of ethanolic extracts of mangrove plants from South East coast of India against chloroquine-sensitive Plasmodium falciparum. Malaria is one of the most prevalent infectious diseases in the world. Treatment for malaria is commonly inadequate due to the lack of quality assured effective drugs. The effectiveness of these drugs is declining at an ever accelerating rate, wit...