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Spectral features of the bound electron acceptor a 2 of photosystem i


, : Spectral features of the bound electron acceptor a 2 of photosystem i. Photochemistry & Photobiology 35(4): 527-532

Difference spectrum for the reduction of A2, a bound secondary electron acceptor of photosystem I, in the thylakoid membranes of a thermophilic blue-green alga, Synechococcus sp., was determined. The experiment was undertaken by subtracting the difference spectrum of P700 photooxidation from the difference spectrum for flash-induced absorption changes, due to oxidation of P700 and reduction of A2, or by measuring light-induced absorption changes under reducing conditions in which reduced A2 accumulates. The spectrum showing a broad bleaching with 2 maxima at 420 and 440 nm indicates that A2 is an Fe-S center different from P430.

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