EurekaMag.com logo
+ Translate

Spectral parameters of the interaction of various ligands with lm 2 and lm 4 forms of highly purified cytochrome p 450


, : Spectral parameters of the interaction of various ligands with lm 2 and lm 4 forms of highly purified cytochrome p 450. Bioorganicheskaya Khimiya 6(5): 757-764

The interaction of various ligands with highly purified LM2 and LM4 forms of [rat liver] cytochrome P-450 was studied by difference spectrophotometry. The Kd were determined for type I ligands (benzene, naphthalene, cyclohexane, dimethylaniline) as well as for type II (aniline, pyridine, imidazole) and modified type II ligands (ethanol, isopropanol). The results obtained were discussed from the viewpoint of ligand action on the spin equilibrium of the cytochrome P-450 forms. The models of high and low spin forms of cytochrome P-450 LM4 in free state and in ligand-bound complexes were proposed.

(PDF 0-2 workdays service)

Accession: 006459739

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Lemberg R.; Cutler M.E., 1970: The anaerobic interaction of ferro cytochrome c with the ferric and oxygenated forms of purified cytochrome c oxidase part 16 cytochrome oxidase and its derivatives. Biochimica et Biophysica Acta 197(1): 1-10

Jurtshuk P.Jr; Yang T.Y., 1979: Spectral characteristics of a highly purified cytochrome oxidase isolated from azotobacter vinelandii. Abstracts of the Annual Meeting of the American Society for Microbiology (79): 158

Adali, O.; Arinç, E., 1990: Electrophoretic, spectral, catalytic and immunochemical properties of highly purified cytochrome P-450 from sheep lung. 1. Cytochrome P-450LgM2 was purified from sheep lung microsomes in the presence of detergents, Emulgen 913 and cholate. 2. The purification procedure involved the chromatography of the detergent solubilized microsomes on DEAE-cellulose and hydroxy...

Akhrem, A.A.; Kiselev, P.A.; Kisel', M.A.; Usanov, S.A.; Metelitsa, D.I., 1979: Effect of phospholipids on the spectral characteristics of highly purified cytochrome P-450 from rabbit liver microsomes. Doklady Akademii Nauk SSSR 245(1): 234-238

Vatsis K.P.; Chiang Y.L.; Coon M.J., 1977: Properties of 2 highly purified forms of liver microsomal cytochrome p 450. Federation Proceedings 36(3): 832

Steele, C.M.; Masson, H.A.; Battershill, J.M.; Gibson, G.G.; Ioannides, C., 1983: Metabolic activation of paracetamol by highly purified forms of cytochrome P-450. The metabolic activation of paracetamol (acetaminophen) to reactive intermediate(s) which bind covalently to proteins was studied in reconstituted systems, employing highly purified preparations of cytochromes P-450 and P-448 isolated from the liv...

Liu, C.Y.; Webster, D.A., 1974: Spectral characteristics and inter conversions of the reduced oxidized and oxygenated forms of purified cytochrome o. Journal of Biological Chemistry 249(13): 4261-4266

Azari M.R.; Wiseman A., 1982: Spectral comparisons of benzo a pyrene binding to cytochrome p 450 p 448 in microsomal fractions and in highly purified form. Biochemical Society Transactions 10(2): 134-135

Akhrem A.A.; Kiselev P.A.; Kisel' M.A.; Usanov S.A.; Metelitsa D.I., 1979: Effect of phospho lipids on spectral characteristics of highly purified cytochrome p 450 of rabbit liver microsomes. Doklady Biophysics 245: 49-52

Dean W.L.; Coon M.J., 1976: Immunochemical properties of 2 highly purified forms of liver microsomal cytochrome p 450 p 450 lm 2 and p 450 lm 4. Federation Proceedings 35(7): 916