+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Spectral properties and chromophore rotation of phytochrome bound to substituted sepharose

, : Spectral properties and chromophore rotation of phytochrome bound to substituted sepharose. Physiologia Plantarum 66(2): 185-190

Brushite purified phytochrome from Avena sativa L. cv. Sol II was bound to phenyl Sepharose, octyl Sepharose, CNBr-activated Sepharose and to anti-phytochrome immunoglobulins immobilized on Sepharose. The spectral properties of phytochrome bound to anti-phytochrome immunoglobulins and to phenyl Sepharose were similar to phytochrome in solution. Phytochrome bound to CNBr-activated Sepharose or to octyl Sepharose showed reduced Pfr formation after red irradiation. The reversal to Pr with far-red light was only partial but a further increase at 667 nm took place slowly in the dark. A peak at 657 nm was seen in the difference spectrum between CNBr-activated Sepharose-bound phytochrome kept in darkness and the identical sample immediately after a far-red irradiation. The change in linear dichroism at 660 nm and 730 nm, induced by plane polarized red or far-red light, was measured. It was computed that the log-wavelength transition moment of phytochrome had an average rotation angle of or The substrate used for immobilization had a limited effect on the rotation angle. Phytochrome immobilized on CNBr-activated Sepharose gave an angle of and phytochrome immobilized on phenyl Sepharose gave an angle of

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006459748

DOI: 10.1111/j.1399-3054.1986.tb02406.x

PDF Full Text: Spectral properties and chromophore rotation of phytochrome bound to substituted sepharose

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Bean, J.C., 1985: Strained-layer epitaxy of germanium-silicon alloys. Despite the dominant position of silicon in semiconductor electronics, its use is ultimately limited by its incompatibility with other semiconducting materials. Strained-layer epitaxy overcomes problems of crystallographic compatibility and produc...

Fodge, D. W.; Anderson, D. M.; Pettey, T. M., 1996: Hemicellulase active at extremes of pH and temperature and utilizing the enzyme in oil wells. Official Gazette of the United States Patent & Trademark Office Patents 1190(1): 161, Sept 3

Cronau, C., 1884: Die Fasenen. Pflege und Aufzucht. Unknown

Simpkins, A.A., 2005: Method for estimating ingestion doses to the public near the Savannah River site following an accidental atmospheric release. At the Savannah River Site, emergency response computer models are used to estimate dose following releases of radioactive materials to the environment. Downwind air and ground concentrations and their associated doses from inhalation and ground s...

Goto Y.; Slinker B.K.; Lewinter M.M., 1990: Dual effects of coronary blood flow and perfusion pressure on left ventricular contractility. Japanese Circulation Journal 54(7): 901

Zalewski, D.; Szczepanski, W., 2004: Contribution of particular features of red deer (Cervus elaphus L.) antlers in the CIC score. A study was conducted in Olsztyn Province, Poland to assess the antlers of the Mazurian red deer (Cervus elaphus) using solely the CIC point scale, and determine the effect of morphological parameters on the quality of antlers collected from stags...

Lovelock B. G., 1990: Recollections of a trip to Santa Lucia. Santa Lucia is one of several islands forming an active volcanic island arc system in the eastern Carribean Sea. Geothermal exploration on the island is focussed near an area of intense solfataric activity, near the town of Soufriere. A brief acco...

Meyers Philip A.; Robinson Rebecca S., 2002: Carbonate and organic carbon contents of sediments from Site 1087, southern Cape Basin. Proceedings of the Ocean Drilling Program, Scientific Results (CD-Rom) 175(2002

Spillner, G.; Schlosser, V., 1976: Surgical therapy of chronic and acute coronary insufficiency. Zfa. Zeitschrift für Allgemeinmedizin 52(29): 1470-1478

Hartnagel, D.; Bichot, A.; Roumes, C., 2008: Eye position affects audio-visual fusion in darkness. We investigated the frame of reference involved in audio-visual (AV) fusion over space. This multisensory phenomenon refers to the perception of unity resulting from visual and auditory stimuli despite their potential spatial disparity. The extent...