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Spectral properties and chromophore rotation of phytochrome bound to substituted sepharose






Physiologia Plantarum 66(2): 185-190

Spectral properties and chromophore rotation of phytochrome bound to substituted sepharose

Brushite purified phytochrome from Avena sativa L. cv. Sol II was bound to phenyl Sepharose, octyl Sepharose, CNBr-activated Sepharose and to anti-phytochrome immunoglobulins immobilized on Sepharose. The spectral properties of phytochrome bound to anti-phytochrome immunoglobulins and to phenyl Sepharose were similar to phytochrome in solution. Phytochrome bound to CNBr-activated Sepharose or to octyl Sepharose showed reduced Pfr formation after red irradiation. The reversal to Pr with far-red light was only partial but a further increase at 667 nm took place slowly in the dark. A peak at 657 nm was seen in the difference spectrum between CNBr-activated Sepharose-bound phytochrome kept in darkness and the identical sample immediately after a far-red irradiation. The change in linear dichroism at 660 nm and 730 nm, induced by plane polarized red or far-red light, was measured. It was computed that the log-wavelength transition moment of phytochrome had an average rotation angle of 31.5.degree. or 180.degree.-31.5.degree. The substrate used for immobilization had a limited effect on the rotation angle. Phytochrome immobilized on CNBr-activated Sepharose gave an angle of 27.8.degree. and phytochrome immobilized on phenyl Sepharose gave an angle of 32.6.degree.

Accession: 006459748

DOI: 10.1111/j.1399-3054.1986.tb02406.x

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