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Spectral properties of 3 quaternary arrangements of pseudomonas aeruginosa pilin


, : Spectral properties of 3 quaternary arrangements of pseudomonas aeruginosa pilin. Biochemistry 22(15): 3640-3646

P. aeruginosa possess multisubunit, filamentous appendages called pili which are involved in adhesion, twitching motility and phage adsorption. The spectral properties of 3 forms of pili were compared. These are native pili, pilin dimers in octyl glycoside and an in vitro assembled form of pilin which called reassembled pilin filaments. Alkaline pH titrations, solvent perturbation, quenching of tryptophan fluorescence with acrylamide and circular dichroism were used to demonstrate that tyrosines-24 and -27 are at a dimer/dimer interface in native pili and in the reassembled pilin filaments. Dissociation of pili by octyl glucoside results in exposure of the 2 tyrosines and in partial exposure of at least 1 tryptophan in pilin.

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Oguri, M.; Iwasaki, I.; Matsumura, T.; Kuroume, T.; Kanbe, Y.; Yamada, T., 1971: Congenital sensitization. II. Food allergy in the aetiology of eczema in the breast-fed neonatal infant with special reference to the participation of congenital factor. Mothers of 15 breast-fed infants with eczema were subjected to elimination and trial ingestion diets of allergenic foods such as eggs, milk and soya beans. Participation of food allergy in the aetiology of eczema was demonstrated. An important rol...

Karalus, Richard; Campagnari, Anthony, 2000: Moraxella catarrhalis: A review of an important human mucosal pathogen. Moraxella catarrhalis has again been recognized as a significant pathogen. The past decade has witnessed an increased amount of research and understanding of the pathogenesis of the organism. This review will summarize the research pertaining to t...

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Rudzki E.; Rebandel P., 1990: Dermatitis from methyl 2 3 epoxy 3 4 methoxyphenylpropionate. Contact Dermatitis 23(5): 382

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Almeida S.; Junho M. C. B., 2000: Hoegbomite in spinel hornblendites of the Morro do Corisco Mine, Minas Gerais, Brazil. International Geological Congress, Abstracts = Congres Geologique International, Resumes 31: (unpaginated

Anonymous, 2011: In vitro amoebicidal activities of Teucrium polium and T chamaedrys on Acanthamoeba castellanii trophozoites and cysts. Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the m...

Egorov, A. D.; Kuvaev, V. B., 1958: Two interesting fodder plants for northern deer in north-eastern Yakut.. The plants are Chosenia (Salix) macrolepis and Chamaenerium latifolium. The chemical composition of the leaves is compared with that of certain lichens (Cladonia spp.], Betula spp. and Eriophorum vaginatum.

Bo, Xuenong; Liu, Min; Schoepfer, Ralf; Burnstock, Geoffrey, 2001: Characterization and expression of ATP P2X4 receptor from embryonic chick skeletal muscle. Previous pharmacological experiments have indicated the existence of ATP P2X receptors in chick embryonic skeletal muscles. In this study we cloned a P2X4-like cDNA encoding a protein of 385 amino acids, which shares 75% and 76% identity with rat...

Hewson, David J.; Mcnair, Peter J.; Marshall, Robert N., 2001: Aircraft control forces and EMG activity: Comparison of pilots before and after flying training. Background: Skilled performers in most motor tasks tend to require less force, muscle activity, and co-contraction of agonist and antagonist muscles to complete tasks. There have been two previous studies on muscle activation patterns and applied...