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Spectral properties of a longwave absorbing form of proto chlorophyll in seeds of cyclanthera explodens

Physiologia Plantarum 48(2): 297-301
Spectral properties of a longwave absorbing form of proto chlorophyll in seeds of cyclanthera explodens
The in vivo absorbance spectrum in the inner seed coat of C. explodens Naud. showed a main peak in the red region at 671 nm and a weak shoulder at about 640 nm. The pigments were extracted with acetone, separated by paper chromatography and analyzed spectrophotometrically. The only detectable pigment was protochlorophyll. The in vivo fluorescence emission spectra had 2 main peaks, 1 at 632 and 1 at 691 nm. The relation between the 2 peaks was changed when the excitation wavelength was altered from 440 and 460 nm. Excitation at 420 nm gave an additional fluorescence emission peak at 595 nm. These data indicate the presence of at least 3 forms of protochlorophyll in the Cyclanthera seed coat. The spectrum of circular dichroism had a very intense and characteristic signal in the red region with a negative asymmetrical Cotton effect [664 (+), 669 (0) and 687 (-) nm]. This indicates that at least one of the protochlorophyll forms is present in a more or less crystalline form.

Accession: 006459761

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