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Spectral properties of phyco erythrin


, : Spectral properties of phyco erythrin. Photosynthetica (Prague) 13(1): 21-28

Absorption and fluorescence spectra as well as degrees of polarization, yield and lifetime of fluorescence of several solutions of phycoerythrin [isolated from Ceramium rubrum] at various pH and concentrations with and without addition of ions were measured. Only 1 type of phycoerythrin chromophore contributed to emission. The concentration depolarization and self-quenching curves were non-typical. Addition of ions strongly perturbed phycoerythrin spectra. Phycoerythrourobilin groups showed a low sensitivity to ion addition and an increase of pigment concentration. They are probably better hidden in the protein matrix than groups responsible for the fluorescence emission. The angles between transition moments of absorption and emission of phycoerythrin were evaluated. Fluorescence quenching by ions at higher phycoerythrin concentration was less efficient than at lower concentrations. Lowest degree of polarization and highest efficiency of emission of phycoerythrin were observed at a physiological pH of about 7.

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