EurekaMag.com logo
+ Translate

Spectral properties of the leucine iso leucine valine binding protein and its complexes with substrates


, : Spectral properties of the leucine iso leucine valine binding protein and its complexes with substrates. Soviet Journal of Bioorganic Chemistry 7(1): 35-46

The difference UV, fluorescence, CD [circular dichroism] and Raman spectra were studied for Escherichia coli leucine-isoleucine-valine binding protein (LIV-protein), for LIV-protein depleted from endogeneous substrates (s-LIV-protein), and for complexes of these 2 proteins with leucine and isoleucine. The s-LIV-protein complex shows altered spectral parameters of the aromatic residues (tryptophan and tyrosine) of the proteins. The protein has at least 2 substrate binding sites which differ in their affinity. The high-affinity site contains 1 tryptophan residue and, possibly, 1 tyrosine residue. A hypothetical model for the substrate binding by LIV-protein is suggested.

(PDF 0-2 workdays service)

Accession: 006459807

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Nabiev I.; Trakhanov S.; Surin A.; Vorotyntseva T.; Efremov E.; Pletnev V., 1982: Optical spectroscopy study of substrate binding by leucine specific and leucine iso leucine valine binding proteins from escherichia coli. Voelter, W , Et Al (Ed ) Chemistry Of Peptides And Proteins, Vol 1; Proceedings Of The 3rd Ussr-Frg Symposium; Makachkala, Ussr, Oct 2-6, 1980 Xiii+554p Walter De Gruyter: Berlin, West Germany; New York, N Y , Usa Illus P467-472

Sack, J.S.; Saper, M.A.; Quiocho, F.A., 1989: Periplasmic binding protein structure and function. Refined X-ray structures of the leucine/isoleucine/valine-binding protein and its complex with leucine. The three-dimensional structure of the native unliganded form of the Leu/Ile/Val-binding protein (Mr = 36,700), an essential component of the high-affinity active transport system for the branched aliphatic amino acids in Escherichia coli, has bee...

Saper, M.A.; Quiocho, F.A., 1983: Leucine iso leucine valine binding protein from escherichia coli structure at 3.0 angstrom resolution and location of the binding site. The structure of the leucine, isoleucine, valine-binding protein, an integral part of the high-affinity branched-chain aliphatic amino acid transport system in E. coli, was solved at 3.0-.ANG. resolution by X-ray crystallography. Five isomorphous...

Ovchinnikov-Yu, A.; Aldanova, N.A.; Grinkevich, V.A.; Arzamazova, N.M.; Moroz, I.N.; Nazimov, I.V., 1977: The primary structure of leucine iso leucine valine binding protein from escherichia coli. The complete amino acid sequence of periplasmic protein from E. coli, selectively binding branched aliphatic amino acids such as leucine, isoleucine and valine (LIV-protein), was established. Exhaustive tryptic hydrolysis and splitting of the prot...

Grinkevich, V.A.; Arzamazova, N.M.; Grinkevich-Kh, A.; Akimenko, Z.A.; Moroz, I.N.; Nazimov, I.V.; Aldanova, N.A., 1979: Primary structure of leucine iso leucine valine binding protein from escherichia coli 2. cyanogen bromide peptides. The splitting of carboxymethylated Leu, Ile, Val(LIV)-binding protein from E. coli by CNBr was performed. By gel-filtration, DEAE-chromatography and paper chromatography, 5 individual peptides out of 6 formed on cleavage were isolated. The arrange...

Grinkevich, V.A.; Arzamazova, N.M.; Potapenko, N.A.; Grinkevich-Kh, A.; Kravchenko, Z.B.; Feigina, M.Y. ; Aldanova, N.A., 1979: Primary structure of leucine iso leucine valine binding protein from escherichia coli 1. the peptides of exhaustive tryptic hydrolysis. Exhaustive tryptic digestion of carboxymethylated Leu, IIe, Val (LIV)-binding protein was performed. For the initial separation of hydrolysate, chromatography on Aminex AG 50W .times. 4 resin and subsequent purification of peptides by paper chroma...

Hoshino T.; Nishio K., 1982: Isolation and characterization of a pseudomonas aeruginosa pao mutant defective in the structural gene for the leucine iso leucine valine alanine and threonine binding protein. A mutant of P. aeruginosa PAO which has a defect in the structural gene for a binding protein for leucine, isoleucine, valine, alanine and threonine (LIVAT-binding protein) was isolated and characterized. DL-4-azaleucine was taken up via the high-...

Moroz, I.N.; Grinkevich, V.A.; Arzamazova, N.M.; Potapenko, N.A.; Akimenko, Z.A.; Nazimov, I.V.; Aldanova, N.A., 1980: The primary structure of leucine iso leucine valine binding protein from escherichia coli 3. the peptides of restricted tryptic and limited acidic hydrolysis determination of a di sulfide bridge in the protein molecule. Tryptic hydrolysis of carboxymethylated, maleylated Leu, Ile, Val (LIV)-binding protein from E. coli was performed. By gel-filtration, DEAE-chromatography and paper chromatography, 8 individual peptides were isolated. The complete structures of 6...

Anonymous, 1980: Primary structure of the leucine iso leucine valine binding protein from escherichia coli 3 peptides from selective tryptic and limited acid hydrolyses determination of the di sulfide bridge in the protein molecule

Williams W.L.S.; Freundlich M., 1969: Role of valine transfer rna in regulation of protein and rna synthesis in escherichia coli abstract iso leucine leucine enz valyl transfer rna synthetase valine starved cells. Bacteriological Proceedings 69: 51