EurekaMag.com logo
+ Translate

Spectral properties of the rhod opsin system of the crayfish astacus leptodactylus


, : Spectral properties of the rhod opsin system of the crayfish astacus leptodactylus. Photochemistry & Photobiology 39(3): 379-390

The absorption spectra of the membrane-bound and of the digitonin-solubilized visual pigment of crayfish A. leptodactylus were investigated by conventional spectrophotometry. A method was developed to isolate purified rhabdoms almost entirely free from screening pigments from a single retina. The quantity of isolated and purified rhabdoms from a single retina was sufficient to measure the absorption spectra of the visual pigment. The absorption spectra of the chromoprotein system (R and M) show that the membrane-bound and the digitonin-solubilized visual pigment isomers are stable at 0.degree. C and pH 7.0. Rhodopsin and metarhodopsin are photoreversible under these conditions without any light-induced denaturation. The difference spectra for the chromoprotein isomers and those of different photostationary states yield maximal values for .DELTA.E at 570 and 485 nm. At neutral pH, 0.degree. C, .lambda.max of rhodopsin is 530 nm. Irradiation with light of .lambda. = 630 to 640 nm isomerizes rhodopsin nearly quantitatively to metarhodopsin with .lambda.max, of 500 nm. The molar extinction coefficient of metarhodopsin is greater than that of rhodopsin by a factor of .apprx. 1.41, each measured at its respective .lambda.max. Metarhodopsin can be isomerized to rhodopsin by irradiating at .lambda. < 630 nm. As the absorption spectra of the 2 chromoprotein isomers overlap, only part of the metarhodopsin can be reversed to rhodopsin. The maximal photoreversion can be achieved by irradiating at 460 nm. The stability of the digitonin-solubilized chromoprotein is remarkably dependent on temperature. Warming the digitonin extract of rhabdoms from 0.degree.-20.degree. or 30.degree. C caused a shift of the rhodopsin spectrum to shorter wavelengths (.lambda.max = 485 nm) accompanied by a decrease of E.lambda.maX by .apprx. 30%.

(PDF 0-2 workdays service)

Accession: 006459809

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Michel Villaz M.; Roche C.; Chabre M., 1982: Orientational changes of the absorbing di pole of retinal upon the conversion of rhod opsin to batho rhod opsin lumi rhod opsin and iso rhod opsin. The orientational change of the absorbing dipole of the retinal chromophore in vertebrate rhodopsin (rhodo) upon photo-excitation to bathorhodopsin (batho), lumirhodopsin (lumi) and isorhodopsin (iso), has been studied by polarized absorption and...

Smietana, P..; Wierzbicka, J.., 1999: Species of Branchiobdella Odier, 1823 (Annelida: Clitellata) associated with the crayfish Astacus astacus and Astacus leptodactylus in Poland. Freshwater Crayfish, 12: 349-355

Mackevichiene G., 1980: Some biochemical differences between 2 species of fresh water crayfish astacus astacus and astacus leptodactylus in lithuania. Laurent, P J. Freshwater Crayfish, Vol. 4. International Symposium, Thonon-Les-Bains; France, Aug. 28-31, . Xx 473p. Institut National De La Recherche Agronomique(inra): Thonon-Les-Bains: France. Illus. Maps. P465-470, 1979

Mackevichiene, G., 1979: Some biochemical differences between two species of freshwater crayfish, Astacus astacus L. and Astacus leptodactylus Esch., in Lithuania. Crayfish caught wild in the Lithuanian SSSR were kept in tanks and fed on fish and Chara nidis. At 4 times of year the moulting cycle and some biochemical characteristics were studied. Astacus leptodactylus had a higher accumulation of dry matter...

Burba, A..; Orentas, V.; Bucinskiene, R., 1997: Stock, size composition, diseases and parasites of the crayfish, Astacus astacus, Astacus leptodactylus and Orconectes limosus in Lithuania. Freshwater Crayfish, 11: 213-218

Duris, Z..; Horka, I.., 2001: Occurrence of narrow-clawed crayfish Pontastacus =Astacus leptodactylus in Kravina vicinity K vyskytu raka bahenniho Pontastacus =Astacus leptodactylus Eschscholtz na Kravinsku. Casopis Slezskeho Zemskeho Muzea Serie A Vedy Prirodni, 50: 78-84 (supplement)

Kossakowski, J., 1965: Crayfish Astacus astacus (L.) and Astacus leptodactylus Esch. migrations in Lake loby, Poland. Ekologia Polska, 13: 515-525

Tsukerzis, YM., 1976: Interspecific competition in closely related species of crayfish (Astacus astacus, Astacus leptodactylus, and Pacifastacus leniusculus, Decapoda, Crustacea). Doklady (Proc) Acad Sci USSR (Biol Sci): 2291-6: 283-285

Mickeniene, L., 1981: Effect of different artificial nutrition on the amount of microorganisms of some physiological groups in the content of the stomach of aboriginal crayfish Astacus astacus, Astacus leptodactylus.1. Darbai Lietuvos TSR Mokslu Akademijos Serija C = Trudy Akademiia Nauk Litovskoi SSR Seriia V: ) 75-79

Papadopol M.; Picos C.A.; Diaconu G., 1987: Studies on the energy metabolism of freshwater crayfish astacus astacus and astacus leptodactylus. At the same acclimatization temperature (20.degree. C.), the O consumption of A. leptodactylus was greater than that of A. astacus. Females of both species had reduced O consumption in comparison with the corresponding males. Oxygen consumption wa...