EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Spectral studies of horse heart porphyrin cytochrome c


, : Spectral studies of horse heart porphyrin cytochrome c. Journal of Biological Chemistry 259(11): 6931-6936

Removal of the heme iron from cytochrome c to generate porphyrin cytochrome c relieves the quenching of porphyrin fluorescence and enhances the fluorescence of the single tryptophan residue and the 4 tyrosine residues. The intensity of the porphyrin fluorescence is not perturbed by denaturation of the protein at neutral pH using either urea or guanidine hydrochloride. However, the amplitude of tryptophan fluorescence is increased by these denaturants from 5 to about 85% of a model tryptophan residue using solutions of 2 microM tryptophan. In contrast to cytochrome c, the tryptophan fluorescence amplitude of denatured porphyrin cytochrome c is independent of pH over the range pH 3.0 to 7.4. Acidification of solutions of either native or denatured porphyrin cytochrome c markedly alters both the visible absorbance and fluorescence of the protein consistent with protonation of two pyrrole nitrogens on the porphyrin. Preliminary analysis of the spectral changes occurring in the acid transition suggests the presence of an intermediate form having only one of these two pyrrole nitrogens protonated.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006459930

PMID: 6327700

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

P. Senthil Kumar; A. Senthil Kumar; V. Keerthi; J.N. Goswami; B. Gopala Krishna; A.S. Kiran Kumar, 2011: Chandrayaan-1 observation of distant secondary craters of Copernicus exhibiting central mound morphology Evidence for low velocity clustered impacts on the Moon. Analysis of the Chandrayaan-1 Terrain Mapping Camera image of a 2km x 27km area in the Mare Imbrium region revealed a cluster of thousands of fresh and buried impact craters in the size range of 2 13m. A majority of the large fresh craters with di...

Bollinger H.; Eugster C.H., 1971: Determination of plus epi muscarine in incybe geophylla part 36 report on muscarine and related substances. Helvetica Chimica Acta 54(5): 1332-1335

Lebedeva Verba, M. P.; Golovanov, D. L.; Inozemtsev, S. A., 2009: Microfabrics of Desert soils of Mongolia. The mineralogical composition of coarse fractions and the specificity of micro- and submicromorphology in the zonal sequence of desert soils of Mongolia (pale brown, gray brown, and extremely arid soils) were studied. A comparative analysis of ana...

Anonymous, 2003: Position of the Ameican Dietetic Association: child and adolescent food and nutrition programs. The position statement of the American Dietetic Association (ADA) regarding the provision of child and adolescent food and nutrition programs is presented. Malnutrition has been linked to delayed development and is acknowledged as a major contrib...

Krupova J., 1968: Some methods for serological proof of trichomonas infection in women trichomonas vaginalis. Ceskoslovenska Epidemiologie Mikrobiologie Imunologie 17(3): 162-168

Rjasanowski, I.; Vogt, L.; Michaelis, D.; Keilacker, H.; Kohnert, K., 1993: The frequency of diabetes in children of type 1 diabetic parents. There is little information in the literature about the actual diabetes risk of children of Type 1 diabetic parents. In a follow-up study of 48 children born from 35 Type 1 diabetic parents between 1955 and 1989, we have recorded all data relating...

Carpentieri, U.; Myers, J.; Daeschner, C.W.; Haggard, M.E., 1988: Effects of iron, copper, zinc, calcium, and magnesium on human lymphocytes in culture. The effects of simultaneous changes of calcium, magnesium, iron, copper, and zinc concentrations were evaluated in normal human T and B lymphocytes, cultured in cation-depleted media. Optimal concentrations for thymidine incorporation (TI) in both...

Reethof G., 1978: Turbulence generated noise in pipe flow. Van Dyke, Milton, J V Wehausen And John L Lumley (Ed ) Annual Review Of Fluid Mechanics, Vol 10 Vi+475p Illus Maps Annual Reviews Inc : Palo Alto, Calif , Usa Isbn 0-8234-0710-0 333-367

Nematzadeh, G. A.; Sattari, M.; Valizadeh, A.; Alinejad, A.; Nori, M. Z., 2003: Hybrid rice technology and achievements in Iran. Rice is a second main staple crop after wheat in Iran. More than 600 000 hectares of cultivated land are allocated to rice cultivation. Rice cultivation has had three distinguished periods in Iran: (1) before 1979, when all local rice varieties (t...

Yang, Z.; Kong, L.; Zhang, J.; Xi, S.; Chen, J., 2001: Dimethyl sulfide emission from waterlogged Chinese paddy soils. A study was conducted on two different paddy soils, each historically having two similar fertilizer programmes, to determine the time course emission of dimethyl sulfide (DMS), and temperature influence on DMS emission, both under aerobic and anae...