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Spectrin bands 1 and 2 are antigenically distinct and are not composed of subunits


Archives of Biochemistry and Biophysics 186(1): 1-8
Spectrin bands 1 and 2 are antigenically distinct and are not composed of subunits
Human erythrocyte membranes and freshly isolated spectrin were separated into their constituent peptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The peptides were electrophoresed from slices of such gels into agarose gels containing anti-spectrin antibodies and Triton X-100. In fresh preparations, precipitin arcs were observed only against peptides migrating as bands 1 and 2. Bands 1 and 2 did not cross-react. There were 2 major arcs from band 1 and 1 principal arc from band 2, plus minor splitting of these arcs. None of the band 1 arcs fused with band 2 arcs. In fresh erythrocyte ghosts, only bands 1 and 2 reacted with anti-spectrin; bands 2.1, 3 and 5, in particular, showed no precipitin arcs. In aged ghosts, arcs appeared in the band 3 region; in aged isolated spectrin, arcs appeared in the band 2.1 region; and in trypsin-degraded spectrin, reactive species occurred in all MW classes. Spectrin has no subunits smaller than 220,000 MW. Bands 1 and 2 are immunochemically distinct.

Accession: 006459994

PMID: 75713

DOI: 10.1016/0003-9861(78)90456-3

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