EurekaMag.com logo
+ Translate

Spectrin hemo globin cross linkages associated with in vitro oxidant hyper sensitivity in pathologic and artificially dehydrated red cells


, : Spectrin hemo globin cross linkages associated with in vitro oxidant hyper sensitivity in pathologic and artificially dehydrated red cells. British Journal of Haematology 54(1): 15-28

Protein crosslinkages are apparent at 215,000-250,000 daltons in electrophoregrams of membranes from H2O2 treated erythrocytes. H2O2 is also capable of inducing crosslinkages of identical MW in stage I and II (red) ghosts and in a mixture of purified spectrin and Hb, but not in white ghosts or in either spectrin or Hb alone. Autoradiographic studies using 14C-methemoglobin and 32P-spectrin confirm the involvement of spectrin and Hb in this reaction. The .alpha. chains of both proteins are more reactive than the corresponding .beta. chains: 3 times more reactive in the case of spectrin and 10 times more reactive in Hb. The reaction is almost totally inhibited by NaCN and partially inhibited by N-ethylmaleimide. Direct addition of malondialdehyde to a spectrin-Hb mixture does not result in protein crosslinkage. Metabolic depletion (40 h), in vivo aging and sucrose dehydration of fresh, normal cells enhance the reaction considerably, whereas in vitro rehydration of xerocytes normalizes their H2O2 sensitivity.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006460008

PMID: 6849833

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Kazimir, E.V., 1993: The application of the sample recognition theory for a system of surgical diagnosis in microsurgery. The paper outlines the applicability of the sample recognition theory to process the data obtained with the use of multifiber optical catheters as part of information systems for diagnostics and visualization for microsurgery. A specific example o...

Hilton, H.N.; Graham, J.Dinny.; Clarke, C.L., 2016: Minireview: Progesterone Regulation of Proliferation in the Normal Human Breast and in Breast Cancer: A Tale of Two Scenarios?. Progesterone (P), which signals through the P receptor (PR), is critical in normal development of the breast, but its signaling axis is also a major driver of breast cancer risk. Here we review recent advances in the understanding of P signaling i...

Giordani, G., 1970: Use of eye-glasses in chicken breeding. Avicoltura 39 (9) 103-104

Minty, A.; Caravatti, M.; Robert, B.; Cohen, A.; Daubas, P.; Weydert, A.; Gros, F.; Buckingham, M., 1981: Muscle coding sequences and their regulation during myogenesis: cloning of muscle actin cDNA probes. For a number of years our group has been mainly interested in the regulation of muscle gene expression during myogenesis. Using primary cultures and cell lines we have tried to find out whether the coding sequences for muscle proteins are already...

Aquilina, J.W.; Mckinney, L.; Pacelli, A.; Richman, L.K.; Waters, D.J.; Thompson, I.; Burghardt, W.F.Jr.; Bostwick, D.G., 1998: High grade prostatic intraepithelial neoplasia in military working dogs with and without prostate cancer. BACKGROUND. High grade prostatic intraepithehal neoplasia (PIN) is the most likely precursor of human prostate cancer and is commonly found in men undergoing prostatic needle biopsy for suspected cancer. Recent work has demonstrated that pet dogs,...

Gross, S.; Pitel, P.; Klemperer, M.; Barbosa, J., 1988: Hemophilia and AIDS: the community and the pediatrician. Journal of the Florida Medical Association 75(9): 599-603

Anonymous., 1978: Marine Observers' log. Insects. Mediterranean Sea. Marine Observer, 117-118 No. 261

Siebor, E.; Neuwirth, C., 2012: The new variant of Salmonella genomic island 1 (SGI1-V) from a Proteus mirabilis French clinical isolate harbours blaVEB-6 and qnrA1 in the multiple antibiotic resistance region. The clinical strain of Proteus mirabilis VB1248 isolated from a blood culture in August 29 was multiresistant (i.e. resistant to β-lactams, fluoroquinolones, aminoglycosides and sulphonamides). We searched for the presence of a Salmonella...

Manuel, L., 2012: My rocky road to a new life. Nursing Standard 1987) 26(48): 62-63

Coniglio, J.G.; Popjak, G., 1963: Fatty acid synthesis in cell-free extracts of lactating rat mammary gland. The stimulation of fatty acid synthesis in cell-free extracts of lactating mammary gland by malonate or citrate was studied. Loss of carboxylase activity after storage at 4 degrees C for 1 day or - 11 degrees C for 7 days was reduced by the additi...