EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectro fluorescent detection of malonaldehyde as a measure of lipid free radical damage in response to ethanol potentiation of spinal cord trauma


, : Spectro fluorescent detection of malonaldehyde as a measure of lipid free radical damage in response to ethanol potentiation of spinal cord trauma. Lipids 12(11): 945-950

Studies of the role of free radical damage to the spinal cord following a 400 g-cm impact have suggested an increase in at least 1 free radical product, malonaldehyde, 24-36 h post injury. To investigate further the role of free radical lipid peroxidation in degeneration of the spinal cord following injury, a study of specific lipid fluorescence (SLF) indicative of the double Schiff-base adduct formed by a reaction between malonaldehyde and cellular components was carried out in the presence of ethanol, a known potentiator of free radical lipid peroxidation. The study was carried out in cats who received a 200 g-cm impact 3 h to 23 days prior to sacrifice. Half of the impacted animals received ethanol, 5 ml/kg, prior to injury. These animals were rendered paraplegic; the nonethanol treated animals were neurologically intact. Controls consisting of laminectomies alone or laminectomies with ethanol but without injury were also studied. Spinal cord segments at the impact or laminectomy site were minced and extracted with chloroform-methanol, cleared by centrifugation, and examined in a scanning fluorometer with excitation maximum at 360 nm and emission maxima at 420, 440, 450 and 460 nm. SLF was minimal in cats 3 h and 1 day post injury, but markedly increased at 3 days. By 5 days background levels were again found in all groups. SLF in the alcohol-pretreated impact animals rose to a peak at 7 days, followed by a decline to background by 10 days. The presence of SLF supports a role for free radical lipid peroxidation in the degenerative changes in the spinal cord following injury. The findings of 2 peaks of SLF activity suggest 2 different sites of damage. One site, found acutely after injury, appears in all groups and was associated with reversible changes; the other site is associated with later changes and chronic paraplegia only. The 2 sites could be the gray and white matter.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006460041

PMID: 927047

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Holland, CH.; Palmer, DC., 1974: Bohemograptus, the youngest graptoloid known from the British Silurian sequence. Special Pap Palaeont, 13: 215-236

Saha, N.; Bayoumi, R.A.; E.S.eikh, F.S.; Samuel, A.P.; E.F.dil, I.; E.H.uri, I.S.; Sebai, Z.A.; Sabaa, H.M., 1980: Some blood genetic markers of selected tribes in Western Saudi Arabia. A total of 292 randomly selected subjects belonging to two indigenous Arab tribes (Harbi and Ghamid) and two immigrant tribes (Mograbi and Mowallad), residents in Western Saudi Arabia, have been tested for genetic variants of six blood groups, fou...

Zhou Yan Wen; Y.Z.i Qing; Luo Zuo Jie; Ouyang Ling Li; Lei Min Xiang; Duan Wen Ruo; Liu Hong; Deng Hong Ming; Yang Zheng Hong; Zhang Ying Jian; X.G.o Shi, 2003: Clinical trial of sibutramine in treatment of simple obesity. AIM: To evaluate the efficacy and safety of sibutramine for simple obesity or overweight with body mass index (BMI) no less than 25. METHODS: Double-blind randomized controlled multicenter clinical trial for 8 wk was conducted compared with placeb...

Ordinaire, I.; Simon, A.; Fréalle, E.; Soula, F.; Valat, A-S.; Rouland, V.; Subtil, D.; Dei-Cas, E.; Camus, D.; Delhaes, L., 2005: Real-time quantitative PCR for toxoplasmosis diagnosis. Congenital toxoplasmosis results from foetus contamination by Toxoplasma gondii during pregnancy. It is a frequent and severe condition calling for close monitoring of mothers at risk. During the last decades, numerous advances have been made spec...

Malik. B.S.; Chaudhrv, A.L.; Was, A.P., 1971: Effects of environmental factors on economic traits of Ghokla sheep. I. Effects on body weights. Over a 5-yr period, data were collected on 314 lambs. Weights (in kg) of and 72 resp. were 2.50 and 2.37 at birth, 13.32 and 11.08 at 4 mth, and 22.44 and 19.65 at 12 mth. Differences at 4 and 12 mth were highly significant. Year of birth was a si...

Bergström, S., 2010: The moment of conception does not exist. Has life no beginning?. Lakartidningen 107(4): 160-161

Coller, B.S., 2002: Science and humanism: the twin pillars of medicine. Mount Sinai Journal of Medicine, New York 69(5): 277-279

James, I.G.; Loria-Kanza, Y.; Jones, T.C., 2007: Short-duration topical treatment of tinea pedis using terbinafine emulsion gel: results of a dose-ranging clinical trial. Pimecrolimus, yet another intriguing local immunomodulator, has been approved for use in atopic dermatitis in individuals of more then 2 years of age. However, its indications are now being extended to include a variety of inflammatory dermatoses,...

Hand, J.S.; Whitehill, J.M., 1986: The prevalence of oral mucosal lesions in an elderly population. In a population of 629, 145 elderly subjects had at least one mucosal lesion; 27% of denture wearers had a denture-related lesion. Lesions were found most commonly on the palate, followed by the lips. Increasing age was the only significant factor...

Muñoz, O.; Delporte, C.; Backhouse, N.; Erazo, S.; Negrete, R.; Maldonado, S.; López-Pérez, J.L.; San Feliciano, A., 2000: A new cucurbitacin glycoside from Kageneckia oblonga (Rosaceae). A novel cucurbitacin glycoside was isolated from aerial parts of K. oblonga (collected from Chile in 1994). Its structure was elucidated as 3 beta -( beta -D-glucosyloxy)-16 alpha ,23 alpha -epoxycucurbita-5,24-dien-11-one from spectral data. This...