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Spectro fluorometric determination of thio purines part 1 6 thio guanine


, : Spectro fluorometric determination of thio purines part 1 6 thio guanine. Talanta 23(5): 383-386

6-Thioguanine is oxidized to the corresponding S-oxide by reaction with H2O2 in 50% acetic acid at 50.degree. C for 5 min. The S-oxide is highly fluorescent and a rapid and sensitive fluorometric method of determination of thioguanine at the ng per ml level in water and .mu.g per ml level in serum and urine was developed on the basis of this reaction. The method was used for the determination of thioguanine in blood and urine samples in leukemia patients.

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