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Spectrodensitometric thin layer chromatography scanning in the determination of sichuan zhu jie ginseng sapogenins



Spectrodensitometric thin layer chromatography scanning in the determination of sichuan zhu jie ginseng sapogenins



Acta Academiae Medicinae Sichuan 15(3): 237-242



Zhu-jie-ginseng, the root of Panax japonicus, a Chinese traditional crude drug, has been long used as an analgesic and hemostatic. Zhu-jie-ginseng is mainly produced in Sichuan [China]. The major components of Zhu-jie-ginseng include 4 types of sapogenins. The results of spectrodensitometric TLC [thin layer chromatography] determination of the sapogenin constituents are as follos: oleanolic acid type (90.5%); 20-s-proto-panaxadiol type (2.4%), 20-s-proto-panaxatriol type (5.2%), 3.beta., 6.alpha., 12.beta., 25-tetrahydroxyl-(20s, 24R)-epoxyldammarane type (1.8%). The predominance of the oleanolic acid type coincide with the results of the analysis of Zhu-jie-ginseng grown in Japan. In Sichuan, the percentage of panaxatriol type (5.2%) is higher than that of panaxadiol type (2.4%). The presence of sapogenins was confirmed, these differ from the reports on Japanese Zhu-jie-ginseng. The simplicity and precision of spectrodensitometric TLC scanning in the analysis of crude drugs was confirmed.

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