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Spectrofluorometric determination of epoxide hydrolase activity


, : Spectrofluorometric determination of epoxide hydrolase activity. Voprosy Meditsinskoi Khimii 32(1): 37-39

A spectrofluorometric procedure is developed for estimation of epoxide hydrolase activity using styrol oxide as a substrate. The data were plotted, reflecting the dependence of the enzymatic reaction on time, pH value and the substrate concentration. Activity of epoxide hydrolase was studied in various tissues and subcellular fractions of rat liver tissue.

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Miyata, M.; Kudo, G.; Lee, Y.-Hue; Yang, T., J.; Gelboin, H., V.; Fernandez-Salguero, P.; Kimura, S.; Gonzalez, F., J., 1999: Targeted disruption of the microsomal epoxide hydrolase gene. Microsomal epoxide hydrolase is required for the carcinogenic activity of 7,12-dimethylbenz(a)anthracene. Microsomal epoxide hydrolase (mEH) is a conserved enzyme that is known to hydrolyze many drugs and carcinogens, and a few endogenous steroids and bile acids. mEH-null mice were produced and found to be fertile and have no phenotypic abnormalities...

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