+ Translate

Spectrophotometric determination of arginine in proteins

, : Spectrophotometric determination of arginine in proteins. Food Chemistry 15(4): 257-260

A simple, sensitive and rapid spectrophotometric method for the direct determination of arginine in protein hydrolysates is described. It is based on the formation of stable yellow colored species (.lambda.max, 440 nm) on the addition of thymol-sodium hypobromite reagent under alkaline conditions. Beer's law is obeyed in the range 1-13 .mu.g/ml with a percentage recovery range from 98.5-100. The different variables which may interfere in the reaction were studied and the most suitable conditions for quantitative application were determined.

Accession: 006460290

DOI: 10.1016/0308-8146(84)90110-9

Download PDF Full Text: Spectrophotometric determination of arginine in proteins

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Vrat, V., 1952: Spectrophotometric determination of arginine and arginine-containing proteins in sera. Complete details for measuring arginine and arginine-containing proteins using a modification of the Sakaguchi reaction with a spectrophotometer have been descr. Spectrophotometric detns. were made on samples of sera from normal and malignant case...

Luigi Messineo, 1966: Modification of the Sakaguchi reaction Spectrophotometric determination of arginine in proteins without previous hydrolysis. 2,4-Dichloro-1-naphthol in alkaline solution becomes red on standing. It becomes red also upon addition of small concentrations of NaOCl and fades when higher concentrations of NaOCl are added. This instability of an alkaline solution of naphthol...

Satake, K.; Luck, J.M., 1958: The spectrophotometric determination of arginine by the Sakaguchi reaction. Bulletin de la Societe de Chimie Biologique 40(12): 1743-1756

Morelli, B., 1990: Simultaneous derivative spectrophotometric determination of aztreonam and L-arginine in injections. Mixtures of aztreonam, 2S-[2 alpha,3 beta(Z)]-2-[[[1-(2-amino-4-thiazolyl-2- [(2-methyl-4-oxo-1-sulfo-3-azetidinyl) amino]-2-oxo- ethylidine]amino]oxy]-2-methyl-propanoic acid, and L-arginine are assayed by peak-to-baseline, peak-to-peak, and zero...

Sastry, C.S.P.; Tummuru, M.K., 1985: Spectrophotometric determination of tryptophan in proteins. A method was developed for the estimation of tryptophan in protein hydrolysates. 5-Hydroxymethylfurfural resulting from sucrose in acidic conditions forms a pale green condensation product with thioglycolic acid, which on treatment with tryptophan...

Montagnani, A., 1953: Spectrophotometric determination of blood proteins. Minerva Medica 44(17): 522-524

Sastry, C.S.P.; Satyanarayana, P.; Tummuru, M.K., 1985: Spectrophotometric determination of tyrosine in proteins. Tyrosine forms a red coupled product with 4-aminophenazone-sodium metaperiodate reagent in an ammoniacal solution which absorbs at 470 nm. Beer's law is obeyed in the range 1.5-15 mu g/ml. The method is simple, sensitive, reproducible, accura...

Prieto, J., A.; Andrade, F.; Aldamiz-Echevarria, L.; Sanjurjo, P., 2006: Determination of free and total carnitine in plasma by an enzymatic reaction and spectrophotometric quantitation spectrophotometric determination of camitine. Objectives: Carnitine initiates the beta-oxidation of fatty acids and its deficiency is a problem in several metabolic diseases. This work describes a validated quick and simple enzymatic assay for the determination of free and total carnitine in...

Ottis, M.; Malát, M., 1980: Extraction spectrophotometric determination in pharmaceutical analysis. II. New spectrophotometric determination of strychnine nitrate and papaverine and quinine chloride. Ceskoslovenska Farmacie 29(5): 131-133

Jurkeviciúte, J.; Malát, M., 1979: Extractive spectrophotometric determination in pharmaceutical analysis. I. New spectrophotometric determination of Septonex and Ajatin with chromazurol S. Ceskoslovenska Farmacie 28(9-10): 379-381