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Spectrophotometric determination of chloramphenicol using 4 nitrosoantipyrine


, : Spectrophotometric determination of chloramphenicol using 4 nitrosoantipyrine. Egyptian Journal of Pharmaceutical Sciences 26(1-4): 245-252

A new spectrophotometric method for the determination of chloramphenicol in alkaline medium is described. p-Nitrophenol, the alkaline hydrolysis product of chloramphicol reacts with 4-nitrosoantipyrine reagent to give a stable red colour. The method is applied for the analysis of chloramphenicol powder or its pharmaceutical formulations. The method is simple and sensitive and gives accurate results compared with B.P. 73 method.


Accession: 006460329

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Related references

Glombitza K.W., 1967: On the inst spectrophotometric determination of chloramphenicol. Pharm Zentralh 106(7): 441-442

Wahbi A.M.; Abdine H.; Korany M.A.; E.Y.zbi F.A., 1978: Spectrophotometric determination of chloramphenicol sulfacetamide in eye drops. Die Pharmazie 33(11): 721-722

Wahbi, A.M.; Abdine, H.; Korany, M.A.; El-Yazbi, F.A., 1978: Spectrophotometric determination of chloramphenicol-sulphacetamide in eye drops. A rapid method for the determination of chloramphenicol in chloramphenicol-sulphacetamide drops is discussed. The method depends upon precipitating sulphacetamide as its silver salt by a solution of silver nitrate. Chloramphenicol is determined in...

Abdel-Hamid, W.E.; Abuirjeie, M.A., 1987: Spectrophotometric determination of chloramphenicol using orthogonal polynomials. Analyst 112(6): 895-897

Shah, R.C.; Raman, P.V.; Shah, B.M., 1963: Spectrophotometric determination of chloramphenicol and tetracycline hydrochloride in mixtures. Journal of Pharmaceutical Sciences 52: 167-168

Leclercq, S., 1960: Spectrophotometric determination of chloramphenicol in the presence of spectral impurities. Journal de Pharmacie de Belgique 15: 321-326

Devani, M.B.; Shishoo, C.J.; Doshi, K.J.; Shah, A.K., 1981: Spectrophotometric determination of chloramphenicol and its esters in complex drug mixtures. When aromatic nitro compounds are reduced with zinc and calcium chloride and reacted with trisodium pentacyanoaminoferrate they give a purple product having an absorbance maximum between 480 and 540 nm. Applying this reaction, a quantitative metho...

Mahrous, M.S.; Abdel-Khalek, M.M., 1984: Spectrophotometric determination of phenothiazines, tetracyclines and chloramphenicol with sodium cobaltinitrite. A spectrophotometric method for determining some phenothiazines, some tetracyclines and chloramphenicol is described. Chlorpromazine hydrochloride, promazine hydrochloride, promethazine hydrochloride, perphenazine and fluphenazine hydrochloride ar...

Wertheim, A.; Dzierzynska, I., 1955: Comparison of quantitative determination of chloramphenicol using colorimetric, spectrophotometric, and microbiological methods. Medycyna Doswiadczalna i Mikrobiologia 7(4): 427-432

Lanza, P., 1959: On some new esters of chloramphenicol. V. Spectrophotometric determination of some esters of chloramphenicol. Il Farmaco; Edizione Pratica 14: 542-551