+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro

, : Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro. Journal of Immunological Methods 21(1-2): 159-166

An assay was developed in which the degree of in vitro spleen cell-mediated sheep red blood cell (SRBC) hemolysis was quantitated spectrophotometrically (QHS). Immune mouse spleen cells, SRBC and guinea pig complement were incubated at C for 1 h in buffer, centrifuged and the supernatant solutions read at 413 nm. The greatest degree of cell-mediated SRBC hemolysis was observed in day +5 harvested spleens following a day 0, 5% SRBC i.p. injection. A stimulator SRBC dose of 0.2% resulted in maximal stimulation with little non-specific hemolysis. This assay was presented as an alternative to the well established plaque forming and rosette assays.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006460435

PMID: 659900

DOI: 10.1016/0022-1759(78)90232-6

PDF Full Text: Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Park, C.G.; Lee, H.; Choi, J.W.; Lee, S.J.; Kim, S.H.; Lim, H.E., 2010: Non-concurrent dosing attenuates the pharmacokinetic interaction between amlodipine and simvastatin. To explore if non-concurrent amlodipine dosing results in less drug interaction, the pharmacokinetic profiles, safety and efficacy endpoints were assessed following repeated doses of simvastatin, co-administered concurrently or non-concurrently wi...

Ting, S.S.; Ziegler, J.; Fischer, E., 1998: Familial granulomatous arthritis (Blau syndrome) with granulomatous renal lesions. Blau syndrome is a granulomatous disease of the skin, eyes, and joints, usually without visceral involvement. It is inherited in a autosomal dominant manner. The Blau susceptibility locus has been mapped to chromosome 16 p 12-q21. A recent report...

Rocha Olivares, A.; Moser, H.G.offrey; Stannard, J., 2000: Molecular identification and description of pelagic young of the rockfishes Sebastes constellatus and Sebastes ensifer. Five rockfish juveniles (15.0-30.4 mm SL) collected in midwater trawls from offshore banks off the coast of California were identified by using a combination of morphological and molecular characters. All had pigment patterns consistent with membe...

Kleinknecht D.; Verger D.; Mignon F.; Richet G., 1970: Renal failure and acute pancreatitis significance of intra glomerular fibrin deposits. Annales de Medecine Interne 121(1): 17-28

Otsuka, K.; Imanishi, Y.; Tada, Y.; Kawakita, D.; Kano, S.; Tsukahara, K.; Shimizu, A.; Ozawa, H.; Okami, K.; Sakai, A.; Sato, Y.; Ueki, Y.; Sato, Y.; Hanazawa, T.; Chazono, H.; Ogawa, K.; Nagao, T., 2016: Clinical Outcomes and Prognostic Factors for Salivary Duct Carcinoma: A Multi-Institutional Analysis of 141 Patients. Among salivary gland malignancies, the prognosis of salivary duct carcinoma (SDC) is assumed to be the poorest. However, because of its low incidence, reliable survival estimates and prognostic factors based on a large number of patients remain to...

Arya, P.; Khalbuss, W.E.; Monaco, S.E.; Pantanowitz, L., 2011: Salivary duct carcinoma with striking neutrophil-tumor cell cannibalism. Cannibalism of neutrophils by tumor cells has previously been reported in certain carcinomas, lymphoma and melanoma. Tumor cannibalism is believed to serve as a tumor-immune escape mechanism, associated with high-grade aggressive cancers with a si...

Fukushi, K.; Nakasato, N.; Narita, N.; Yoshida, K., 1972: Electron microscopic study of the Auer body. Acta Pathologica Japonica 22(3): 509-515

Nyiendo, J.; Kilbourn, J.P., 1979: Cost-effective clinical microbiology. American Journal of Medical Technology 45(5): 393-396

Li, W-Xian.; Liu, Y.; Li, Y-Jie.; Feng, S-Yan.; Zhang, J.; Ao, B-Yang.; Sun, X-Jun.; Saiji, L., 2013: Synthesis and luminescence properties of two novel lanthanide (III) complexes of acetophenonylcarboxymethyl sulphoxide. A novel ligand containing multiple coordinating groups (sulfinyl, carboxyl and carbonyl groups), acetophenonylcarboxymethyl sulphoxide, was synthesized. Its corresponding two lanthanide (III) binary complexes were synthesized and characterized by...

P.X.ugang; Jiang Jigang, 2000: Using chemical reaction kinetics to calculate the quantity of oil generated in immature source rocks of the Qianjiang Formation in the Jianghan Basin, Hubei, China. Based on the relationship between apparent activation energy (AAE) and hydrocarbon generation rate, frequency factor and AAE, which were derived from pyrolysis of immature source rocks in the study area, a model of chemical reaction kinetics (MCRK...