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Spectrophotometric determination of menadione and menadione sodium bisulfite in pharmaceutical preparations


, : Spectrophotometric determination of menadione and menadione sodium bisulfite in pharmaceutical preparations. International Journal of Pharmaceutics (Kidlington) 39(1-2): 137-140

A simple, selective and sensitive spectrophotometric method is described by the determination of menadione (or menadione sodium bisulfite) in bulk samples and pharmaceutical preparations. The method is based on the reaction of its reduction product with 3-methyl-2-benzothiazolinone hydrozone (MBTH) in the presence of ferric chloride whereby intense blue colour with maximum absorption at 650-670 nm is developed. The reaction is sensitive enough to permit the determination of 0.6-7.5 .mu.g menadione/ml with an average recovery of 99% and a standard deviation of 1.7%. There is no interference from other vitamins or from any common pharmaceutical diluents and additives.

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Accession: 006460442

DOI: 10.1016/0378-5173(87)90208-0

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