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Spectrophotometric determination of total gossypol in cottonseeds and cottonseed meals


, : Spectrophotometric determination of total gossypol in cottonseeds and cottonseed meals. Analytical Chemistry 56(1): 30-32

A new spectrophotometric method for the determination of total gossypol content in cottonseeds and cottonseed meals was developed. The method was based on the reaction of gossypol with 3-amino-1-propanol an its subsequent complexation with Fe(III). The green colored Fe(III)-bis-(aminopropanol)-gossypol complex had a characteristic absorption maximum at 620 nm. The colored system obeyed Beer's law in the concentration range of 4-80 ppm gossypol. The effects of several experimental variables on the determination of gossypol were studied and the stolchlometric composition of the complex was determined. The new method was simple, rapid and precise and yielded results comparable with the standard AOCS [American Oil Chemists' Society] method. The method was applied for the determination of total gossypol in several cottonseed and cottonseed meal samples.

Accession: 006460595

PMID: 6546479

DOI: 10.1021/ac00265a010

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Related references

Botsoglou, N.A.; Kufidis, D.C., 1990: Determination of total gossypol in cottonseed and cottonseed meals by derivative UV spectrophotometry. A new method for the determination of total gossypol in cottonseed and cottonseed meals has been developed. The method involves oxalic acid hydrolysis of the bound gossypol in a methyl ethyl ketone-water azeotrope, partitioning the liberated gossy...

Smith, F.H., 1958: Spectrophotometric determination of total gossypol in cottonseed meal and cottonseed meats. An improved method has been developed for the determination of total gossypol in cottonseed and cottonseed meal. The sample is heated with aniline to convert the gossypol to dianilinogossypol, which is extracted with chloroform and measured spectr...

Botsoglou, Na, 1991: Determination of free gossypol in cottonseed and cottonseed meals by second-derivative ultraviolet spectrophotometry. Journal of agricultural and food chemistry 39(3): 478-482

Botsoglou N.A., 1991: Determination of free gossypol in cottonseed and cottonseed meals by second derivative uv spectrophotometry. A new spectrophotometric method for the determination of "free" gossypol in cottonseed and cottonseed meals has been developed. The method involves extraction of free gossypol with aqueous acetone, hydrolysis of the "soluble-bound&q...

Sherwood, F.W., 1926: Studies on gossypol The gossypol and d-gossypol content of some North Carolina cottonseed meals. The gossypol and [image]-gossypol content of 40 North Carolina cotton-seed meals is reported. In the 22 meals which were made by the continuous cooker process the gossypol varied from 0.007 to 0.228% and the [image]-gossypol from 0.633 to 1.076%....

Hassan, K.; Anwar, A.; Ibrahim, J.I., 1959: Total and free gossypol contents of home-produced cottonseed meals. Undecorticated cottonseed meal, 8 samples, had total gossypol 1.09 to 1.53, average 1.36% and free gossypol 0.07 to 0.36, 0.19%. Two decorticated meals had on average 2.03 and 0.15, respectively. As undecorticated meal contains half as much protei...

Kuhlmann, S.W.; Calhoun, M.C.; Huston, J.E.; Baldwin, B.C.Jr, 1994: Total, - and -gossypol in plasma and liver of lambs fed cottonseed meals processed by three methods. Journal of Dairy Science 77(SUPPL 1): 145

Calk C.B.; Calhoun M.C.; Huston J.E.; Kuhlmann S.W.; Baldwin B.C.Jr; Bales K.W., 1992: Response of lambs to expander solvent cottonseed meals varying in free and total gossypol. Journal of Animal Science 70(SUPPL 1): 33

Hassan, K.; Anwar, A.; Ibrahim, J.I., 1960: Factors affecting total and free gossypol contents of home-produced cottonseed meals. For earlier work see Abst. 245, Vol. 33. Average values for gossypol in 5 samples of each of the varieties Ashmouni, Giza 30, Menoufi, Karnak, representing the range of cottonseed characters, were, in kernels, total 1.93, 1.92, 1.66, 1.65%, free 1...

Pons, W.A., Jr.; Hoffpauir, C.L., 1955: Determination of free gossypol in chemically treated cottonseed meals containing dianilinogossypol. A chemical method for the estimation of free gossypol in meals which may contain dianilinogossypol is described, in which aniline is used in place of p-anisidine. A qualitative test for the presence of dianilinogossypol is also given, since the mo...