EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectrophotometric determination of urea in urine stains on foods and containers


Journal - Association of Official Analytical Chemists 63(5): 985-987
Spectrophotometric determination of urea in urine stains on foods and containers
Urea is extracted from rodent urine-contaminated material with hot acetone. After the extract is evaporated to dryness, aqueous urease solution is added to produce ammonia and CO2. A blue product, indophenol, is formed by the reaction of ammonia with phenol in the presence of hypochlorite. Absorbance is maximum at 625 nm. Presence of urea is easily detected at 4 .mu.g. Detection of urine contamination in various materials is compared with detection by the AOAC [Association of Official Analytical Chemists] urease-H2PtCl6 test.


Accession: 006460613

PMID: 7410309



Related references

Spectrophotometric determination of dulcin p ethoxyphenyl urea in foods. Food Chemistry 12(2): 109-114, 1983

Spectrophotometric determination of urea in blood & urine. Nordisk Medicin 61(12): 471-472, 1959

The quantitative determination of urea in urine spectrophotometric determination following thin-layer chromatographic separation on cellulose sheets. 1965

A simple spectrophotometric method for the determination of urea in blood and urine. Journal of Clinical Pathology 14: 202-204, 1961

The internal lacquering of tinplate containers for foods. I. The determination of tin in foods and a survey of the tin content of some canned foods. J. Counc. sci. industr. Res. Aust, 12: 303-310, 1939

The internal lacquering of tinplate containers for foods. 1. The determination of tin in foods and a snrvey of the tin content of some canned foods. J. Counc. Sci. Indust. Res. Austral, 12: 303-310, 1939

Spectrophotometric determination of L-ascorbic acid in pharmaceutical preparations, foods and urine by formation of a 2-oximinocyclohexanone thiosemicarbazone-iron(II) complex. Analyst 113(11): 1657-1659, 1988

Spectrophotometric determination of L-ascorbic acid in pharmaceutical preparations, foods and urine by formation of a 2-oximinocyclohexanone thiosemicarbazone--iron(II) complex. Analyst 113(11): 1657-1659, 1988

Spectrophotometric determination of carboxyhemoglobin in dry blood stains. Biologicheskii Zhurnal Armenii 39(1): 80-82, 1986

Development of a radial gel diffusion technique for the identification of urea in urine stains. Journal of Forensic Sciences 29(2): 601-606, 1984