EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Spectrophotometric determination of urea in urine stains on foods and containers


, : Spectrophotometric determination of urea in urine stains on foods and containers. Journal - Association of Official Analytical Chemists 63(5): 985-987

Urea is extracted from rodent urine-contaminated material with hot acetone. After the extract is evaporated to dryness, aqueous urease solution is added to produce ammonia and CO2. A blue product, indophenol, is formed by the reaction of ammonia with phenol in the presence of hypochlorite. Absorbance is maximum at 625 nm. Presence of urea is easily detected at 4 .mu.g. Detection of urine contamination in various materials is compared with detection by the AOAC [Association of Official Analytical Chemists] urease-H2PtCl6 test.


Accession: 006460613

PMID: 7410309

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Rao M.V.; Kapur O.P.; Sastry C.S.P., 1983: Spectrophotometric determination of dulcin p ethoxyphenyl urea in foods. A new spectrophotometric method for the determination of dulcin in foods was developed. Dulcin is hydrolyzed with 6N NaOH under reflux: the hydrolyzed dulcin is oxidized at pH 3.0 with sodium hypochlorite and coupled with phenol at pH 10.0 to give...

With, T.K.; Petersen, T.D., 1959: Spectrophotometric determination of urea in blood & urine. Nordisk Medicin 61(12): 471-472

Anonymous, 1965: The quantitative determination of urea in urine spectrophotometric determination following thin-layer chromatographic separation on cellulose sheets

With, T.K.; Petersen, T.D.; Petersen, B., 1961: A simple spectrophotometric method for the determination of urea in blood and urine. Journal of Clinical Pathology 14: 202-204

Lynch, L.J.; Kefford, J.F., 1939: The internal lacquering of tinplate containers for foods. I. The determination of tin in foods and a survey of the tin content of some canned foods. Reduced cream and condensed milk of Australian manufacture packed in non-lacquered cans, were found to contain 64 and 30 mg. of tin per kg. respectively. The reduced cream had stained the tinplate.

Lynch, L.J.; Kefford, J.F., 1939: The internal lacquering of tinplate containers for foods. 1. The determination of tin in foods and a snrvey of the tin content of some canned foods. Examination of the tins and determination of the Sn content of a number of commercial samples of canned fruit juices, soups, fruits and other materials marketed in Australia indicated the desirability of using lacquered tins for fruits and vegetab...

Salinas, F.; Galeano Diaz, T., 1988: Spectrophotometric determination of L-ascorbic acid in pharmaceutical preparations, foods and urine by formation of a 2-oximinocyclohexanone thiosemicarbazone-iron(II) complex. A simple method is proposed for the estimation of L-ascorbic acid in pharmaceutical preparations, foods and urine. The procedure is based on the rapid reduction of the iron(III) ion in the presence of L-ascorbic acid. The Fe(II) formed is estimate...

Salinas, F.; Galeano Díaz, T., 1988: Spectrophotometric determination of L-ascorbic acid in pharmaceutical preparations, foods and urine by formation of a 2-oximinocyclohexanone thiosemicarbazone--iron(II) complex. Analyst 113(11): 1657-1659

Babakhanyan R.V.; Petrov L.V., 1986: Spectrophotometric determination of carboxyhemoglobin in dry blood stains. Biologicheskii Zhurnal Armenii 39(1): 80-82

Bedrosian, J.L.; Stolorow, M.D.; Tahir, M.A., 1984: Development of a radial gel diffusion technique for the identification of urea in urine stains. A radial gel diffusion method utilizing urease and bromthymol blue has been developed for urine stain identification. Urea, present in urine in relatively high concentrations, can be detected from urine stain extracts. This technique provides both...