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Spectrophotometric determination of urea in urine stains on foods and containers


, : Spectrophotometric determination of urea in urine stains on foods and containers. Journal - Association of Official Analytical Chemists 63(5): 985-987

Urea is extracted from rodent urine-contaminated material with hot acetone. After the extract is evaporated to dryness, aqueous urease solution is added to produce ammonia and CO2. A blue product, indophenol, is formed by the reaction of ammonia with phenol in the presence of hypochlorite. Absorbance is maximum at 625 nm. Presence of urea is easily detected at 4 .mu.g. Detection of urine contamination in various materials is compared with detection by the AOAC [Association of Official Analytical Chemists] urease-H2PtCl6 test.

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